Journal ArticleDOI
gyrB as a phylogenetic discriminator for members of the Bacillus anthracis-cereus-thuringiensis group
Reads0
Chats0
TLDR
The gyrB gene proved more highly differential than 16S, while, at the same time, as analytical as costly and laborious DNA:DNA hybridization techniques in differentiating species within the B. cereus group.About:
This article is published in Journal of Microbiological Methods.The article was published on 2004-03-01. It has received 134 citations till now. The article focuses on the topics: Cereus & Bacillus cereus.read more
Citations
More filters
Journal ArticleDOI
Bacillus thuringiensis: a genomics and proteomics perspective.
TL;DR: This review highlights what the authors consider the most relevant issues and topics pertaining to the genomics and proteomics of Bt, the most widely used environmentally compatible biopesticide worldwide.
Journal ArticleDOI
Comparison of gyrB gene sequences, 16S rRNA gene sequences and DNA-DNA hybridization in the Bacillus subtilis group.
TL;DR: It is concluded that the gyrB gene may be an efficient alternative target for identification and taxonomic analysis of members of the B. subtilis group and provide higher resolution than 16S rRNA gene sequences.
Journal ArticleDOI
WST-1-based cell cytotoxicity assay as a substitute for MTT-based assay for rapid detection of toxigenic Bacillus species using CHO cell line.
TL;DR: The WST-1-based cytotoxicity assay could be used as an alternative method to MTT assay for rapid (3 h) confirmation of toxigenic Bacillus species in foods prior to their retail distribution or consumption.
Journal ArticleDOI
Bacillus safensis sp. nov., isolated from spacecraft and assembly-facility surfaces.
TL;DR: Thirteen strains of a novel spore-forming, Gram-positive, mesophilic heterotrophic bacterium were isolated from spacecraft surfaces and assembly-facility surfaces at the Jet Propulsion Laboratory and the Kennedy Space Center, revealing distinct clustering of the tested strains into two distinct species of the genus Bacillus.
Journal ArticleDOI
Microbiomes of the dust particles collected from the International Space Station and Spacecraft Assembly Facilities
Aleksandra Checinska,Alexander J. Probst,Parag Vaishampayan,James R. White,Deepika Kumar,Victor G. Stepanov,George E. Fox,Henrik R. Nilsson,Duane L. Pierson,Jay L. Perry,Kasthuri Venkateswaran +10 more
TL;DR: The results of this study provide strong evidence that specific human skin-associated microorganisms make a substantial contribution to the ISS microbiome, which is not the case in Earth-based cleanrooms.
References
More filters
Journal ArticleDOI
A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences.
TL;DR: Some examples were worked out using reported globin sequences to show that synonymous substitutions occur at much higher rates than amino acid-altering substitutions in evolution.
Journal ArticleDOI
Report of the Ad Hoc Committee on Reconciliation of Approaches to Bacterial Systematics
Lawrence G. Wayne,Don J. Brenner,R. R. Colwell,Patrick A. D. Grimont,O. Kandler,Micah I. Krichevsky,L. H. Moore,W. E. C. Moore,R. G. E. Murray,Erko Stackebrandt,M. P. Starr,H. G. Truper +11 more
Book
Methods for general and molecular bacteriology
TL;DR: Methodology for General and Molecular Microbiology Morphology Light microscopy Determinative and cytological light microscopy Electron microscopy Cell fractionation Antigen-antibody reactions Growth: Physicochemical factors in growth Nutrition and media Enrichment and isolation Solid, liquid/solid and semisolid culture Liquid culture Growth measurement Culture preservation Molecular Genetics: Gene mutation Gene transfer in Gram-negative bacteria Gene transferIn Gram-positive bacteria Plasmids Transposon mutagenesis
Journal ArticleDOI
Bacillus anthracis, Bacillus cereus, and Bacillus thuringiensis—One Species on the Basis of Genetic Evidence
Erlendur Helgason,Ole Andreas Økstad,Dominique A. Caugant,Henning Johansen,Agnès Fouet,Michèle Mock,Ida K. Hegna,Anne-Brit Kolstø +7 more
TL;DR: It is shown by multilocus enzyme electrophoresis and by sequence analysis of nine chromosomal genes that B. anthracis should be considered a lineage of B. cereus and this determination is not only a formal matter of taxonomy but may also have consequences with respect to virulence and the potential of horizontal gene transfer within the B. Cereus group.
Journal ArticleDOI
The genome sequence of Bacillus anthracis Ames and comparison to closely related bacteria
Timothy D. Read,Timothy D. Read,Scott N. Peterson,Scott N. Peterson,Nicolas J. Tourasse,Les Baillie,Les Baillie,Ian T. Paulsen,Ian T. Paulsen,Karen E. Nelson,Hervé Tettelin,Derrick E. Fouts,Jonathan A. Eisen,Jonathan A. Eisen,Steven R. Gill,Erik Holtzapple,Ole Andreas Økstad,Erlendur Helgason,Jennifer Rilstone,Martin Wu,James F. Kolonay,Maureen J. Beanan,Robert J. Dodson,Lauren M. Brinkac,Michelle L. Gwinn,Robert T. DeBoy,Ramana Madpu,Sean C. Daugherty,A. Scott Durkin,Daniel H. Haft,William C. Nelson,Jeremy Peterson,Mihai Pop,Hoda Khouri,Diana Radune,Jonathan L. Benton,Yasmin Mahamoud,Lingxia Jiang,Ioana R. Hance,Janice Weidman,Kristi Berry,Roger D. Plaut,Alex M. Wolf,Kisha Watkins,William C. Nierman,Alyson Hazen,Robin T. Cline,Caroline Redmond,Joanne Elizabeth Thwaite,Owen White,Steven L. Salzberg,Steven L. Salzberg,Brendan Thomason,Arthur M. Friedlander,Theresa M. Koehler,Philip C. Hanna,Anne-Brit Kolstø,Claire M. Fraser,Claire M. Fraser +58 more
TL;DR: Several chromosomally encoded proteins that may contribute to pathogenicity—including haemolysins, phospholipases and iron acquisition functions—and numerous surface proteins that might be important targets for vaccines and drugs are found.
Related Papers (5)
PCR amplification and direct sequencing of gyrB genes with universal primers and their application to the detection and taxonomic analysis of Pseudomonas putida strains.
S Yamamoto,Shigeaki Harayama +1 more
The neighbor-joining method: a new method for reconstructing phylogenetic trees.
Naruya Saitou,Masatoshi Nei +1 more