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Journal ArticleDOI

gyrB as a phylogenetic discriminator for members of the Bacillus anthracis-cereus-thuringiensis group

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TLDR
The gyrB gene proved more highly differential than 16S, while, at the same time, as analytical as costly and laborious DNA:DNA hybridization techniques in differentiating species within the B. cereus group.
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This article is published in Journal of Microbiological Methods.The article was published on 2004-03-01. It has received 134 citations till now. The article focuses on the topics: Cereus & Bacillus cereus.

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Bacillus thuringiensis: a genomics and proteomics perspective.

TL;DR: This review highlights what the authors consider the most relevant issues and topics pertaining to the genomics and proteomics of Bt, the most widely used environmentally compatible biopesticide worldwide.
Journal ArticleDOI

Comparison of gyrB gene sequences, 16S rRNA gene sequences and DNA-DNA hybridization in the Bacillus subtilis group.

TL;DR: It is concluded that the gyrB gene may be an efficient alternative target for identification and taxonomic analysis of members of the B. subtilis group and provide higher resolution than 16S rRNA gene sequences.
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WST-1-based cell cytotoxicity assay as a substitute for MTT-based assay for rapid detection of toxigenic Bacillus species using CHO cell line.

TL;DR: The WST-1-based cytotoxicity assay could be used as an alternative method to MTT assay for rapid (3 h) confirmation of toxigenic Bacillus species in foods prior to their retail distribution or consumption.
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Bacillus safensis sp. nov., isolated from spacecraft and assembly-facility surfaces.

TL;DR: Thirteen strains of a novel spore-forming, Gram-positive, mesophilic heterotrophic bacterium were isolated from spacecraft surfaces and assembly-facility surfaces at the Jet Propulsion Laboratory and the Kennedy Space Center, revealing distinct clustering of the tested strains into two distinct species of the genus Bacillus.
References
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Journal ArticleDOI

A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences.

TL;DR: Some examples were worked out using reported globin sequences to show that synonymous substitutions occur at much higher rates than amino acid-altering substitutions in evolution.
Book

Methods for general and molecular bacteriology

TL;DR: Methodology for General and Molecular Microbiology Morphology Light microscopy Determinative and cytological light microscopy Electron microscopy Cell fractionation Antigen-antibody reactions Growth: Physicochemical factors in growth Nutrition and media Enrichment and isolation Solid, liquid/solid and semisolid culture Liquid culture Growth measurement Culture preservation Molecular Genetics: Gene mutation Gene transfer in Gram-negative bacteria Gene transferIn Gram-positive bacteria Plasmids Transposon mutagenesis
Journal ArticleDOI

Bacillus anthracis, Bacillus cereus, and Bacillus thuringiensis—One Species on the Basis of Genetic Evidence

TL;DR: It is shown by multilocus enzyme electrophoresis and by sequence analysis of nine chromosomal genes that B. anthracis should be considered a lineage of B. cereus and this determination is not only a formal matter of taxonomy but may also have consequences with respect to virulence and the potential of horizontal gene transfer within the B. Cereus group.
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The genome sequence of Bacillus anthracis Ames and comparison to closely related bacteria

TL;DR: Several chromosomally encoded proteins that may contribute to pathogenicity—including haemolysins, phospholipases and iron acquisition functions—and numerous surface proteins that might be important targets for vaccines and drugs are found.
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