PatentDOI
Hematopoietic stem cells
Reads0
Chats0
TLDR
In this paper, human hemotopoietic stem cells are characterized as CD34-Lin and CD34 -CD38-Lin, and methods of isolating and using such cells in compositions and in methods for the reconstitution of a deficient or missing cell population.Abstract:
The present invention relates to human hemotopoietic stem cells characterized as CD34-Lin- and CD34-CD38-Lin- and to methods of isolating and using such cells in compositions and in methods for the reconstitution of a deficient or missing cell population. The present invention also provides a population of human hematopoietic stem cells that can be isolated and genetically altered for introduction in a human patient, to correct various genetic disorders and cultured and further differentiated in vitro to provide a new population of cells.read more
Citations
More filters
Journal ArticleDOI
Ex vivo expansion of hematopoietic stem cells derived from umbilical cord blood in rotating wall vessel.
TL;DR: It is demonstrated that the RWV bioreactor can provide a better environment for UCB MNCs expansion, enhance the contact between HSCs and accessory cells and make the utilization of cytokines more effective than T-flask.
Journal ArticleDOI
A paradoxical role for IFN-γ in the immune properties of mesenchymal stem cells during viral challenge
Helen S. Kang,Misha Habib,Jennifer L. Chan,Christina Abavana,Julius A. Potian,Nicholas M. Ponzio,Pranela Rameshwar +6 more
TL;DR: This report shows that in cases where MSC are used in transplantation for repair of damaged tissue, they can exert an additional role by protecting the host to viral challenges and thereby protect from its immunosuppressive properties.
Patent
Hematopoietic cells from human embryonic stem cells
TL;DR: In this paper, a system for producing cells of the hematopoietic lineage from embryonic stem cells was proposed, which was conducted in the presence of hematogenic cytokines and other factors listed in the disclosure.
Patent
Using undifferentiated embryonic stem cells to control the immune system
TL;DR: In this paper, the patient is administered with undifferentiated embryonic stem cells or early progenitor cells, which induces a state of inflammatory quiescence or immune unresponsiveness, which in turn enhances engraftment of cells derived from the same stem cell line given for purposes of regenerative medicine.
Journal ArticleDOI
Stem sense: a proposal for the classification of stem cells.
TL;DR: It is suggested that the effort to establish a consensus on what constitutes a tissue-specific stem cell (definition) and how one would stratify cells (classification) would greatly facilitate progress and perhaps help resolve some of the outstanding controversies.
References
More filters
Journal ArticleDOI
Isolation and functional properties of murine hematopoietic stem cells that are replicating in vivo.
TL;DR: It is discovered that display of Hoechst fluorescence simultaneously at two emission wavelengths revealed a small and distinct subset of whole bone marrow cells that had phenotypic markers of multipotential HSC, which were shown in competitive repopulation experiments to contain the vast majority of HSC activity from murine bone marrow and to be enriched at least 1,000-fold for in vivo reconstitution activity.
Journal ArticleDOI
Purification and Characterization of Mouse Hematopoietic Stem Cells
TL;DR: Mouse bone marrow hematopoietic stem cells were isolated with the use of a variety of phenotypic markers and thirty of these cells are sufficient to save 50 percent of lethally irradiated mice, and to reconstitute all blood cell types in the survivors.
Journal ArticleDOI
Long-Term Lymphohematopoietic Reconstitution by a Single CD34-Low/Negative Hematopoietic Stem Cell
TL;DR: A monoclonal antibody raised to the mouse homolog of CD34 (mCD34) was used to purify mouse HSCs to near homogeneity to enable analysis of the self-renewal and multilineage differentiation of individual HSCS.
Journal ArticleDOI
Stem cells: units of development, units of regeneration, and units in evolution.
TL;DR: The review begins with a detailed examination of hematopoi-via the umbilical vein to the fetal liver between dpc 8.5 etic (blood-forming) stem cells, and the transcription pro-The earliest stem cells in ontogeny are totipotent, ex- files of each of these populations are quite distinct.
Journal ArticleDOI
Chromosomal translocations in human cancer.
TL;DR: Fusion proteins formed after chromosomal translocations are common in a range of tumour types; these are unique tumour antigens and are therefore potential targets for therapy design.