Heterogeneity of human colony-forming cells (CFU-C): differences in size, rate of colony formation, and responsiveness to colony-stimulating factor.
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The results indicate that human bone marrow CFU-C's are heterogeneous and that the two purified CSF fractions from human lung have different CF U-C specificity.About:
This article is published in Journal of Laboratory and Clinical Medicine.The article was published on 1978-07-01 and is currently open access. It has received 26 citations till now. The article focuses on the topics: Cell morphology & Population.read more
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The nature and action of granulocyte-macrophage colony stimulating factors
Antony W. Burgess,Donald Metcalf +1 more
TL;DR: Information on the regulation of GM-CSF production, on the modulators of its action on specific target cells, and on its role in vivo will be required before the physiologic function of this molecule can be properly assessed.
Journal ArticleDOI
Separation of functionally distinct human granulocyte-macrophage colony-stimulating factors.
TL;DR: Mixing and titration experiments indicated that the differences in functional specificities of the two GM-CSFs were not due to the presence of specific inhibitory molecules or lower absolute levels of CSF in one fraction relative to the other.
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Activities of four purified growth factors on highly enriched human hematopoietic progenitor cells.
Annabel Strife,C Lambek,David Wisniewski,Subhash C. Gulati,Judith C. Gasson,David W. Golde,Karl Welte,Janice Gabrilove,Bayard D. Clarkson +8 more
TL;DR: It is clear from these results that additional factor(s) are present in MoCM that are necessary to stimulate CFU-GM, BFU-E, andCFU-GEM maximally in vitro.
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The production of myeloid blood cells and their regulation during health and disease.
TL;DR: The proposed review characterizes these biomolecules biochemically and functionally, including receptor-ligand interactions and the secondary messengers within the cell which mediate their functional activity, under conditions of hematopoietic disorders and under normal conditions.
References
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The growth of mouse bone marrow cells in vitro
TR Bradley,Donald Metcalf +1 more
TL;DR: A simple in vitro technique for the growth of colonies from single cell suspensions of mouse bone marrow involves the plating of marrow cells in agar on feeder layers of other cells, those from 8-day-old mouse kidney and 17th day mouse embryo being shown to be the most efficient types of feeder layer.
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Separation of cells by velocity sedimentation.
TL;DR: It is shown, using an electronic cell counter and pulse height analyzer, that cells are fractionated according to size across the gradient such that the sedimentation velocity approximately equals r2/4 where r is the cell radius in microns.
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Physical separation of hemopoietic stem cells from cells forming colonies in culture.
TL;DR: Mouse bone marrow cells in suspension were separated into a number of fractions on the basis of cell density by equilibrium density gradient centrifugation, or on the based of cell size by velocity sedimentation, to demonstrate that cells in some fractions formed more colonies in vivo than in the culture system.
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A low molecular weight factor in lung-conditioned medium stimulating granulocyte and monocyte colony formation in vitro.
J. W. Sheridan,D. Metcalf +1 more
TL;DR: Medium conditioned by excised whole lungs from endotoxin‐injected C57BL mice was highly active in stimulating hemopoietic colony formation, particularly of granulocytic type, in agar cultures of mouse bone marrow cells.
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Heterogeneity of in vitro colony‐ and cluster‐forming cells in the mouse marrow: Segregation by velocity sedimentation
D. Metcalf,H. R. Macdonald +1 more
TL;DR: Partial segregation of colony‐forming cells was observed according to the morphological types of colonies generated, large cells tending to generate macrophage colonies and small cells, granulocytic colonies, which were more responsive to stimulation by CSF than small cells.