Heterogeneity of phospholipid composition in the bacterial membrane.
Anne N. Tucker,David C. White +1 more
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Heterogeneity in the distribution or binding of the membrane phospholipids was demonstrated in the membrane fragments released from Haemophilus parainfluenzae by treatment with ethylenediaminetetraacetic acid (EDTA)-tris(hydroxymethyl)-aminomethane (Tris).Abstract:
Heterogeneity in the distribution or binding of the membrane phospholipids was demonstrated in the membrane fragments released from Haemophilus parainfluenzae by treatment with ethylenediaminetetraacetic acid (EDTA)-tris(hydroxymethyl)-aminomethane (Tris). The membrane fragments released early in the EDTA-Tris treatment contained two- to fivefold higher proportions of cardiolipin and phosphatidylglycerol and less phosphatidylethanolamine as well as phospholipids with threefold lower specific activity of the phospholipid phosphate after a short pulse of 32P than were found in the residue. Heterogeneity was best demonstrated with shorter EDTA-Tris treatments and shorter periods of growth with 32P. EDTA-Tris treatment appeared to progressively strip phospholipids from the cells that were synthesized at progressively later times.read more
Citations
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Effect of Phospholipase C Hydrolysis of Membrane Phospholipids on Membranous Enzymes
TL;DR: Results show the heterogeneity of membranous enzymes with respect to their dependence upon the presence of intact membrane phospholipids, and the similarity in the rate of hydrolysis of phospholips and the rates of inactivation of certain membran Mous enzymes suggests that these enzymes are dependent on phospholipopids whose susceptibility to phospholIPase C hydrolytic is similar to the bulk of membrane phosphols.
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References
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David C. White,Anne N. Tucker +1 more
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Lipid composition of the electron transport membrane of Haemophilus parainfluenzae.
TL;DR: Three distinct classes of phospholipids are separable according to their relative fatty acid compositions, and the fatty acid composition of the cardiolipin is intermediate between the other two classes.
Journal ArticleDOI
Indentification and Localization of the Fatty Acids in Haemophilus parainfluenzae
David C. White,Richard H. Cox +1 more
TL;DR: Haemophilus parainfluenzae was capable of synthesizing 22 fatty acids, which were equivalent to 4% of the bacterial dry weight and were localized in the membrane-wall complex, which contained the respiratory pigments, the quinone, and the phospholipids.