Heterology expression of the Arabidopsis C-repeat/dehydration response element binding factor 1 gene confers elevated tolerance to chilling and oxidative stresses in transgenic tomato.
Tsai Hung Hsieh,Jent Turn Lee,Pei Tzu Yang,Li Hui Chiu,Yee-yung Charng,Yu Chie Wang,Ming-Tsair Chan +6 more
TLDR
Results from the current study suggest that heterologous CBF1expression in transgenic tomato plants may induce several oxidative-stress responsive genes to protect from chilling stress.Abstract:
In an attempt to improve stress tolerance of tomato (Lycopersicon esculentum) plants, an expression vector containing an Arabidopsis C-repeat/dehydration responsive element binding factor 1 (CBF1) cDNA driven by a cauliflower mosaic virus 35S promoter was transferred into tomato plants. Transgenic expression of CBF1 was proved by northern- and western-blot analyses. The degree of chilling tolerance of transgenic T1 and T2 plants was found to be significantly greater than that of wild-type tomato plants as measured by survival rate, chlorophyll fluorescence value, and radical elongation. The transgenic tomato plants exhibited patterns of growth retardation; however, they resumed normal growth after GA3 (gibberellic acid) treatment. More importantly, GA3-treated transgenic plants still exhibited a greater degree of chilling tolerance compared with wild-type plants. Subtractive hybridization was performed to isolate the responsive genes of heterologous Arabidopsis CBF1 in transgenic tomato plants. CATALASE1 (CAT1) was obtained and showed activation in transgenic tomato plants. The CAT1 gene and catalase activity were also highly induced in the transgenic tomato plants. The level of H2O2 in the transgenic plants was lower than that in the wild-type plants under either normal or cold conditions. The transgenic plants also exhibited considerable tolerance against oxidative damage induced by methyl viologen. Results from the current study suggest that heterologous CBF1 expression in transgenic tomato plants may induce several oxidative-stress responsive genes to protect from chilling stress.read more
Citations
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Plant responses to drought, salinity and extreme temperatures: towards genetic engineering for stress tolerance
TL;DR: The present review summarizes the recent advances in elucidating stress-response mechanisms and their biotechnological applications and examines the following aspects: regulatory controls, metabolite engineering, ion transport, antioxidants and detoxification, late embryogenesis abundant (LEA) and heat-shock proteins.
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Transcriptional regulatory networks in cellular responses and tolerance to dehydration and cold stresses.
TL;DR: This review article highlights transcriptional regulation of gene expression in response to drought and cold stresses, with particular emphasis on the role of transcription factors and cis-acting elements in stress-inducible promoters.
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Cold stress regulation of gene expression in plants.
TL;DR: This work focuses on recent progress in transcriptional, post-transcriptional and post- translational regulation of gene expression that is critical for cold acclimation in temperate plants.
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ICE1: a regulator of cold-induced transcriptome and freezing tolerance in Arabidopsis
Viswanathan Chinnusamy,Masaru Ohta,Siddhartha Kanrar,Byeong-ha Lee,Xuhui Hong,Manu Agarwal,Jian-Kang Zhu +6 more
TL;DR: The identification of ICE1 (inducer of CBF expression 1), an upstream transcription factor that regulates the transcription of CBFs genes in the cold, and its overexpression in wild-type plants enhances the expression of the CBF regulon in thecold and improves freezing tolerance of the transgenic plants.
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Understanding and Improving Salt Tolerance in Plants
TL;DR: Genetic engineering of ion transporters and their regulators, and of the CBF (C-repeat-binding factor) regulons, holds promise for future development of salt-tolerant crops.
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