Histological identification of Helicobacter pylori: comparison of staining methods.
TLDR
The modified Giemsa stain is the method of choice because it is sensitive, cheap, easy to perform, and reproducible, and when H pylori are present, careful examination will almost always reveal them.Abstract:
Aim—To determine whether two recently described staining methods (the modified McMullen9s and the Helicobacter pylori silver stain HpSS methods) used for the histological identification of H pylori organisms are superior to two established techniques (the modified Giemsa and anti-H pylori antibody immunostain) in terms of availability, reproducibility, rapidity, sensitivity, and cost. Methods—Histological sections from 63 paired gastric biopsies from adult patients previously investigated for dyspepsia were stained with the four methods and these were assessed blindly and independently by two observers. Of the 63 patients, 30 were originally negative in all tests for H pylori infection, 30 were positive, and the remaining three cases had discordant results using a combination of five tests (rapid biopsy urease test, urea breath test, culture, serology, and histology). Results—Interobserver agreement was best with the antibody method (98%), followed by the McMullen9s (90%), Giemsa (87%), and HpSS (85%). Of the 60 “gold standard” positive and negative cases, 30 were positive by the modified Giemsa stain, 29 by the McMullen9s method, 29 by HpSS, and 30 by the antibody stain. However, there were two false positives with the HpSS method. The modified Giemsa is the cheapest and easiest to perform technically. Conclusions—When H pylori are present, careful examination will almost always reveal them, whichever of these stains is used. However, the modified Giemsa stain is the method of choice because it is sensitive, cheap, easy to perform, and reproducible.read more
Citations
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Diagnosis of Helicobacter pylori infection: Current options and developments
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TL;DR: The aim of this article is to review the current options and novel developments of diagnostic tests and their applications in different clinical conditions or for specific purposes.
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Diagnosis of Helicobacter pylori: invasive and non-invasive tests.
TL;DR: Helicobacter pylori infection can be diagnosed by invasive techniques requiring endoscopy and biopsy and by non-invasive techniques, such as serology, the urea breath test, urine/blood or detection of H.pylori antigen in stool specimen.
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Diagnosis of Helicobacter pylori: what should be the gold standard?
TL;DR: PCR based diagnosis may be considered as gold standard by designing primers extremely specific to H. pylori and targeting at least more than one conserved genes, and specificity of PCR may be improved by use of internal Primers.
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Canadian Helicobacter Study Group Consensus Conference: Update on the approach to Helicobacter pylori infection in children and adolescents--an evidence-based evaluation.
Nicola L. Jones,Philip M. Sherman,Carlo A Fallone,Nigel Flook,Fiona Smaill,Sander Veldhuyzen van Zanten,Richard H. Hunt,Alan B. R. Thomson +7 more
TL;DR: An evidence-based appraisal of 14 topics was undertaken in a consensus conference to update guidelines based on the best available evidence using an established and uniform methodology to address and formulate recommendations for each topic.
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