Journal ArticleDOI
Host-derived proteinases and degradation of dentine collagen in situ.
TLDR
In this article, the presence and activity of matrix metalloproteinases 1, 2 and 9 was investigated in an in situ study of the dentine root caries.Abstract:
Dentine root caries is a process of demineralization and degradation of the organic matrix by proteinases. In this in situ study, the presence and activity of the matrix metalloproteinases 1, 2 and 9read more
Citations
More filters
Journal ArticleDOI
Dental adhesion review: Aging and stability of the bonded interface
Lorenzo Breschi,Annalisa Mazzoni,Alessandra Ruggeri,Milena Cadenaro,Roberto Di Lenarda,E. Dorigo +5 more
TL;DR: This study critically discusses the latest peer-reviewed reports related to formation, aging and stability of resin bonding, focusing on the micro and nano-phenomena related to adhesive interface degradation.
Journal ArticleDOI
Collagen Degradation by Host-derived Enzymes during Aging
David H. Pashley,Franklin R. Tay,Cky Yiu,Mamoru Hashimoto,Lorenzo Breschi,Ricardo M. Carvalho,Shuichi Ito +6 more
TL;DR: It is hypothesized that collagen degradation occurred over time, via host-derived matrix metalloproteinases that are released slowly over time through proteolytic enzyme inhibitors or mineral oil.
Journal ArticleDOI
Limitations in Bonding to Dentin and Experimental Strategies to Prevent Bond Degradation
Yan Liu,Leo Tjäderhane,Lorenzo Breschi,Annalisa Mazzoni,Nan Li,Jing Mao,David H. Pashley,Franklin Chi Meng Tay +7 more
TL;DR: Five experimental strategies developed by different research groups for extending the longevity of resin-dentin bonds are examined, finding a combination of several of these strategies should result in overcoming the critical barriers to progress currently encountered in dentin bonding.
Journal ArticleDOI
Chlorhexidine Arrests Subclinical Degradation of Dentin Hybrid Layers in vivo
TL;DR: Self-destruction of collagen matrices occurs rapidly in resin-infiltrated dentin in vivo and may be arrested with the use of chlorhexidine as an MMP inhibitor.
Journal ArticleDOI
Matrix Metalloproteinases and Other Matrix Proteinases in Relation to Cariology: The Era of ‘Dentin Degradomics'
TL;DR: It is presented the case that the organic matrix in caries-affected dentin may not be preserved as intact as previously considered, and this would seriously compromise the matrix ability for intrafibrillar remineralization, which is considered essential in restoring the dentin's mechanical properties.
References
More filters
Journal ArticleDOI
Electrophoretic analysis of plasminogen activators in polyacrylamide gels containing sodium dodecyl sulfate and copolymerized substrates.
Christa Heussen,Eugene B. Dowdle +1 more
TL;DR: A new technique is described for the electrophoretic analysis of plasminogen activators in sodium dodecyl sulfate-polyacrylamide gels containing copolymerized pl asminogen and gelatin, which can be used to detect as little as 1 mU of urokinase and effectively distinguishes between melanoma- and u rokinase-type plasmineg activators.
Journal ArticleDOI
The Activation and Function of Host Matrix Metalloproteinases in Dentin Matrix Breakdown in Caries Lesions
TL;DR: Results demonstrate the pH-dependent activation mechanism of MMPs, which may have a distinct role in different physiological and pathological conditions and demonstrate that host M MPs, activated by bacterial acids, have a crucial role in the destruction of dentin by caries.
Journal ArticleDOI
Tetracyclines inhibit connective tissue breakdown: new therapeutic implications for an old family of drugs.
TL;DR: Evidence is presented that this property of tetracycline may be therapeutically useful in retarding pathologic connective tissue breakdown, including bone resorption, and in the treatment of periodontal and several medical diseases.
Journal ArticleDOI
A simple method to determine nanogram levels of 4-hydroxyproline in biological tissues.
TL;DR: It is shown that the calculation of recoveries based on the external standards method using either pyrrole or hydroxyproline could be subject to considerable error and necessitated the use of the method of standard additions.
Journal ArticleDOI
Matrix metalloproteinases and their inhibitors in gingival crevicular fluid and saliva of periodontitis patients
Tuula Ingman,Taina Tervahartiala,Y. Ding,Harald Tschesche,Ahmad Haerian,Denis F. Kinane,Yrjö T. Konttinen,Timo Sorsa +7 more
TL;DR: Tests based on specific antibodies against PMN MMPs, especially MMP-8, might serve as a reliable method of measuring and monitoring enzyme levels in GCF from different periodontitis patients.