Human Adult Stem Cells Maintain a Constant Phenotype Profile Irrespective of Their Origin, Basal Media, and Long Term Cultures
Indumathi Somasundaram,Rashmi Mishra,Harikrishnan Radhakrishnan,Rajkumar sankaran,Venkata Naga Srikanth Garikipati,Dhanasekaran Marappagounder +5 more
TLDR
It is understood from the study that immunophenotyping acts as a valuable tool to identify inherent property of each cell, thereby leading to a valuable cell based therapy.Abstract:
The study aims to identify the phenotypic marker expressions of different human adult stem cells derived from, namely, bone marrow, subcutaneous fat, and omentum fat, cultured in different media, namely, DMEM-Low Glucose, Alpha-MEM, DMEM-F12 and DMEM-KO and under long term culture conditions (>P20). We characterized immunophenotype by using various hematopoietic, mesenchymal, endothelial markers, and cell adhesion molecules in the long term cultures (Passages-P1, P3, P5, P9, P12, P15, and P20.) Interestingly, data revealed similar marker expression profiles irrespective of source, basal media, and extensive culturing. This demonstrates that all adult stem cell sources mentioned in this study share similar phenotypic marker and all media seem appropriate for culturing these sources. However, a disparity was observed in the markers such as CD49d, CD54, CD117, CD29, and CD106, thereby warranting further research on these markers. Besides the aforesaid objective, it is understood from the study that immunophenotyping acts as a valuable tool to identify inherent property of each cell, thereby leading to a valuable cell based therapy.read more
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Comparative Characterization of Cells from the Various Compartments of the Human Umbilical Cord Shows that the Wharton’s Jelly Compartment Provides the Best Source of Clinically Utilizable Mesenchymal Stem Cells
TL;DR: The results show that when isolating MSCs from the UC, the WJ should be the preferred compartment, and a standardized method of derivation must be used so as to make meaningful comparisons of data between research groups.
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Identity, proliferation capacity, genomic stability and novel senescence markers of mesenchymal stem cells isolated from low volume of human bone marrow
Gabrielis Kundrotas,Evelina Gasperskaja,Grazina Slapsyte,Zivile Gudleviciene,Jan Krasko,Ausra Stumbryte,Regina Liudkeviciene +6 more
TL;DR: Characteristics of MSCs isolated from residual human bone marrow transplantation material and expanded to clinically relevant numbers at passages 3-4 and 6-7 indicated that early passage hBM-MSCs are genomically stable and retain identity and high proliferation capacity.
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Revisiting MSC expansion from critical quality attributes to critical culture process parameters
Céline Martin,Eric Olmos,Marie-Laure Collignon,Natalia de Isla,Fabrice Blanchard,Isabelle Chevalot,Annie Marc,Emmanuel Guedon +7 more
TL;DR: This analysis of the recent literature once again shows the intrinsic relationship between critical quality attributes (CQA) of hMSC expanded for cell therapy and critical process parameters used during their expansion.
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Optimal culture conditions are critical for efficient expansion of human testicular somatic and germ cells in vitro
Itai Gat,Itai Gat,Leila Maghen,Melissa Filice,Brandon A. Wyse,Khaled Zohni,Keith Jarvi,Kirk C. Lo,Andrée Gauthier Fisher,Clifford Librach,Clifford Librach +10 more
TL;DR: Use of DMEM-F12 reduces TSC proliferation while preserving their unique characteristics, leading to improved germ cell aggregates formation compared with StemPro-34, the standard basal medium used in the majority of previous reports.
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Therapeutic Benefit for Late, but Not Early, Passage Mesenchymal Stem Cells on Pain Behaviour in an Animal Model of Osteoarthritis.
Victoria Chapman,Hareklea Markides,Devi Rani Sagar,Luting Xu,James J. Burston,Paul I. Mapp,Alasdair G. Kay,Robert H. Morris,Oksana Kehoe,Alicia J. El Haj +9 more
TL;DR: Late passage MSCs and Kenalog attenuated established pain behaviour in MNX rats, but did not alter MNX-induced joint pathology at the end of the study period.
References
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Journal ArticleDOI
Minimal criteria for defining multipotent mesenchymal stromal cells. The International Society for Cellular Therapy position statement
Massimo Dominici,K. Le Blanc,Ingo Mueller,I. Slaper-Cortenbach,Frank C. Marini,Diane S. Krause,Robert J. Deans,Armand Keating,Darwin J. Prockop,Edwin M. Horwitz +9 more
TL;DR: The Mesenchymal and Tissue Stem Cell Committee of the International Society for Cellular Therapy proposes minimal criteria to define human MSC, believing this minimal set of standard criteria will foster a more uniform characterization of MSC and facilitate the exchange of data among investigators.
Journal ArticleDOI
Multilineage cells from human adipose tissue: implications for cell-based therapies.
Patricia A. Zuk,Min Zhu,Hiroshi Mizuno,Jerry I. Huang,Futrell Jw,Adam J. Katz,Prosper Benhaim,H. P. Lorenz,Marc H. Hedrick +8 more
TL;DR: The data support the hypothesis that a human lipoaspirate contains multipotent cells and may represent an alternative stem cell source to bone marrow-derived MSCs.
Journal ArticleDOI
Bone Marrow Origin of Endothelial Progenitor Cells Responsible for Postnatal Vasculogenesis in Physiological and Pathological Neovascularization
Takayuki Asahara,Haruchika Masuda,Tomono Takahashi,Christoph Kalka,Christopher Pastore,Marcy Silver,Marianne Kearne,Meredith Magner,Jeffrey M. Isner +8 more
TL;DR: Findings indicate that postnatal neovascularization does not rely exclusively on sprouting from preexisting blood vessels (angiogenesis); instead, EPCs circulate from bone marrow to incorporate into and thus contribute to postnatal physiological and pathological neov vascularization, which is consistent with postnatal vasculogenesis.
Journal ArticleDOI
Adipose-Derived Stem Cells for Regenerative Medicine
TL;DR: The isolation, characterization, and preclinical and clinical application of adipose-derived stem cells (ASCs) are reviewed in this article.
Journal ArticleDOI
Comparison of Multi-Lineage Cells from Human Adipose Tissue and Bone Marrow
Daniel A. De Ugarte,Kouki Morizono,Amir Elbarbary,Zeni Alfonso,Patricia A. Zuk,Min Zhu,Jason L. Dragoo,Peter Ashjian,Bert Thomas,Prosper Benhaim,Irvin S. Y. Chen,John K. Fraser,Marc H. Hedrick +12 more
TL;DR: This study is the first comparison of PLA cells and MSCs isolated from the same patient, and no significant differences were observed for yield of adherent stromal cells, growth kinetics, cell senescence, multi-lineage differentiation capacity, and gene transduction efficiency.