Journal ArticleDOI
Human erythrocyte Ca2+-Mg2+-ATPase: mechanism of stimulation by Ca2+.
Thomas J. Lynch,Wai Yiu Cheung +1 more
TLDR
The mode of stimulation of human erythrocyte Ca2+-Mg2--ATPase by calmodulin appears similar to that of Ca2-dependent adenylate cyclase and phosphodiesterase.About:
This article is published in Archives of Biochemistry and Biophysics.The article was published on 1979-04-15. It has received 112 citations till now. The article focuses on the topics: Calmodulin & Ca2+/calmodulin-dependent protein kinase.read more
Citations
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Journal ArticleDOI
Calmodulin plays a pivotal role in cellular regulation
TL;DR: The role of calcium ions (Ca2+) in cell function is beginning to be unraveled at the molecular level as a result of recent research on calcium-binding proteins and particularly on calmodulin.
Journal ArticleDOI
Purified (Ca2+-Mg2+)-ATPase of the erythrocyte membrane. Reconstitution and effect of calmodulin and phospholipids.
TL;DR: The purified and the reconstituted forms of the enzyme displayed the properties expected of the intact Ca2+ pump; they had an appropriate (Ca2+-Mg2+)-ATPase activity which displayed a relatively low affinity for Ca2+.
Journal ArticleDOI
Acidic phospholipids, unsaturated fatty acids, and limited proteolysis mimic the effect of calmodulin on the purified erythrocyte Ca2+ - ATPase.
TL;DR: The isolated enzyme, after reconstitution in phosphatidylcholine liposomes in the absence of calmodulin, can be activated by limited proteolysis and the trypsinized enzyme has the same high Vmax and high affinity for Ca2+ of the enzyme in the presence of Calmodulin.
Journal ArticleDOI
Purification and characterization of two forms of a low-affinity Ca2+-ATPase from erythrocyte membranes
Stellan Hjertén,Huazhen Pan +1 more
TL;DR: The results obtained can be explained by assuming that the two proteins corresponding to the two bands obtained in SDS electrophoresis have a similar structure and can associate to complexes A and B and cause misinterpretation of the data obtained.
References
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Journal Article
Protein Measurement with the Folin Phenol Reagent
TL;DR: Procedures are described for measuring protein in solution or after precipitation with acids or other agents, and for the determination of as little as 0.2 gamma of protein.
Journal ArticleDOI
Identification of the Ca2+-dependent modulator protein as the fourth subunit of rabbit skeletal muscle phosphorylase kinase.
Philip Cohen,Ann Burchell,J.Gordon Foulkes,Patricia T.W. Cohen,Thomas C. Vanaman,Anagus C. Nairin +5 more
TL;DR: The finding that the ‘modulator’ could substitute for troponin-C in restoring calcium sensitivity to actomyosin ATPase in reconstituted systems was substantiated by the determination of the amino acid sequence of the “calcium-dependent modulator” from bovine brain and rat testis.
Journal ArticleDOI
Cyclic 3',5'-nucleotide phosphodiesterase. Evidence for and properties of a protein activator.
TL;DR: It was concluded that the residual activity associated with the purified enzyme was due to an incomplete removal of the activator from the enzyme.
Journal ArticleDOI
Modulator protein as a component of the myosin light chain kinase from chicken gizzard.
TL;DR: It is shown that the modulator protein from smooth muscle and troponin C are distinct proteins, and the similarity of the 17 000 kinase component and the modulators protein with respect to amino acid composition, absorption spectrum, and electrophoresis in urea-polyacrylamide gels.
Journal ArticleDOI
Phosphodiesterase protein activator mimics red blood cell cytoplasmic activator of (Ca2+-Mg2+)ATPase.
TL;DR: Both phosphodiesterase activator and RBC cytoplasmic activator increase the Vmax and the apparent affinity of (Ca2-Mg2+)ATPase for Ca2+.