Journal ArticleDOI
Calmodulin plays a pivotal role in cellular regulation
TLDR
The role of calcium ions (Ca2+) in cell function is beginning to be unraveled at the molecular level as a result of recent research on calcium-binding proteins and particularly on calmodulin.Abstract:
The role of calcium ions (Ca2+) in cell function is beginning to be unraveled at the molecular level as a result of recent research on calcium-binding proteins and particularly on calmodulin. These proteins interact reversibly with Ca2+ to form a protein . Ca2+ complex, whose activity is regulated by the cellular flux of Ca2+. Many of the effects of Ca2+ appear to be exerted through calmodulin-regulated enzymes.read more
Citations
More filters
Journal ArticleDOI
Calbindin D-28k and parvalbumin in the rat nervous system
TL;DR: The distribution of structures stained with mono- and polyclonal antibodies to the calcium-binding proteins calbindin D-28k and parvalbumin in the nervous system of adult rats is described and it can be stated that cal bindin antibodies mainly label cells with thin, unmyelinated axons projecting in a diffuse manner.
Journal ArticleDOI
Calcium-binding proteins in the nervous system.
TL;DR: Among the many calcium-binding proteins in the nervous system, parvalbumin, calbindin-D28K and calretinin are particularly striking in their abundance and in the specificity of their distribution.
Journal ArticleDOI
Calcium: A Central Regulator of Plant Growth and Development
TL;DR: Today no one questions the assertion that Ca2+ is a crucial regulator of growth and development in plants.
Journal ArticleDOI
Protein-binding assays in biological liquids using microscale thermophoresis.
TL;DR: The affinity of the small-molecule inhibitor quercetin to its kinase PKA was determined in buffer and human serum, revealing a 400-fold reduced affinity in serum, which may allow to make more reliable conclusions on protein functionality, and may facilitate more efficient drug development.
Journal ArticleDOI
Ca2+-induced hydrophobic site on calmodulin: application for purification of calmodulin by phenyl-Sepharose affinity chromatography.
TL;DR: It is apparent that phenyl-SepHarose offers several advantages over phenothiazine-Sepharose for affinity purification of calmodulin, and the time required for this procedure is substantially less than that of previously described procedures.
References
More filters
Journal ArticleDOI
Adenosine 3',5'-phosphate in biological materials. I. Purification and properties of cyclic 3',5'-nucleotide phosphodiesterase and use of this enzyme to characterize adenosine 3',5'-phosphate in human urine.
R.W. Butcher,Earl W. Sutherland +1 more
TL;DR: Data indicated a significant role for the enzyme in the control of the levels of adenosine 3’,5’-phosphate present in biological systems, which was very desirable because of the extremely low levels present in most biological materials.
Journal ArticleDOI
Carp Muscle Calcium-binding Protein II. STRUCTURE DETERMINATION AND GENERAL DESCRIPTION
TL;DR: The structure of crystalline carp muscle calcium-binding protein (parvalbumin) has been determined by x-ray diffraction techniques to nominal 1.85-A resolution and the electron density map is interpreted in terms of the 108 amino acid sequence described in Paper I in this series.
Journal ArticleDOI
Prostaglandins and thromboxanes.
TL;DR: This chapter discusses major advances in prostaglandin chemistry and biological activity and indicates the extent to which research has progressed towards the realization of earlier hopes of the discovery of new therapeutic agents.