Journal ArticleDOI
Human mesenchymal stem cells support megakaryocyte and pro-platelet formation from CD34(+) hematopoietic progenitor cells.
TLDR
A coculture system of hMSCs and CD34+ cells in serum‐free media without exogenous cytokines is established and results suggest that MSCs residing within the megakaryocytic microenvironment in bone marrow provide key signals to stimulatemegakaryocyte and platelet production from CD34- hematopoietic cells.Abstract:
Megakaryocytopoiesis and thrombocytopoiesis result from the interactions between hematopoietic progenitor cells, humoral factors, and marrow stromal cells derived from mesenchymal stem cells (MSCs) or MSCs directly. MSCs are self-renewing marrow cells that provide progenitors for osteoblasts, adipocytes, chondrocytes, myocytes, and marrow stromal cells. MSCs are isolated from bone marrow aspirates and are expanded in adherent cell culture using an optimized media preparation. Culture-expanded human MSCs (hMSCs) express a variety of hematopoietic cytokines and growth factors and maintain long-term culture-initiating cells in long-term marrow culture with CD34(+) hematopoietic progenitor cells. Two lines of evidence suggest that hMSCs function in megakaryocyte development. First, hMSCs express messenger RNA for thrombopoietin, a primary regulator for megakaryocytopoiesis and thrombocytopoiesis. Second, adherent hMSC colonies in primary culture are often associated with hematopoietic cell clusters containing CD41(+) megakaryocytes. The physical association between hMSCs and megakaryocytes in marrow was confirmed by experiments in which hMSCs were copurified by immunoselection using an anti-CD41 antibody. To determine whether hMSCs can support megakaryocyte and platelet formation in vitro, we established a coculture system of hMSCs and CD34(+) cells in serum-free media without exogenous cytokines. These cocultures produced clusters of hematopoietic cells atop adherent MSCs. After 7 days, CD41(+) megakaryocyte clusters and pro-platelet networks were observed with pro-platelets increasing in the next 2 weeks. CD41(+) platelets were found in culture medium and expressed CD62P after thrombin treatment. These results suggest that MSCs residing within the megakaryocytic microenvironment in bone marrow provide key signals to stimulate megakaryocyte and platelet production from CD34(+) hematopoietic cells.read more
Citations
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Journal ArticleDOI
Human mesenchymal stem cells modulate allogeneic immune cell responses
TL;DR: Insight is offered into the interactions between allogeneic MSCs and immune cells and mechanisms likely involved with the in vivo MSC-mediated induction of tolerance that could be therapeutic for reduction of GVHD, rejection, and modulation of inflammation.
Journal ArticleDOI
Mesenchymal Stem Cells
TL;DR: The bone marrow contains multipotent MSC, which can be easily isolated and cultured in vitro, and the possibility of their clinical use in cell and gene therapy is analyzed.
Journal ArticleDOI
Mesenchymal Stem Cells in the Wharton's Jelly of the Human Umbilical Cord
Hwai Shi Wang,Shih-Chieh Hung,Shu Tine Peng,Chun Chieh Huang,Hung Mu Wei,Yi Jhih Guo,Yu Show Fu,Mei Chun Lai,Chin Chang Chen +8 more
TL;DR: It is shown that mesenchymal cells isolated from the umbilical cord have multilineage potential and that, under suitable culture conditions, are able to differentiate into cells of the adipogenic and osteogenic lineages.
Journal ArticleDOI
Mesenchymal stem cells
TL;DR: This work will review the information dealing with the biology of mesenchymal progenitors as it has been revealed mainly by ex vivo studies performed with bone marrow-derived cells.
Journal ArticleDOI
Primary murine MSC show highly efficient homing to the bone marrow but lose homing ability following culture.
TL;DR: It is suggested that in vitro propagation of BM-derived MSC dramatically decreases their homing to BM and spleen, as well as the seeding fraction in the BM was reduced and after transplantation of 48 h cultured primary MSC no CFU-F were detected in the lymphohematopoietic organs.
References
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Multilineage Potential of Adult Human Mesenchymal Stem Cells
Mark F. Pittenger,Alastair Morgan Mackay,Stephen C. Beck,Rama K. Jaiswal,Robin Douglas,Joseph D. Mosca,Mark Aaron Moorman,Donald William Jr. Ward Road Simonetti,Stewart Craig,Daniel R. Marshak +9 more
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Journal ArticleDOI
Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease.
TL;DR: In this article, the rat pancreas RNA was used as a source for the purification of alpha-amylase messenger ribonucleic acid (RBA) using 2-mercaptoethanol.
Journal ArticleDOI
Mesenchymal stem cells
TL;DR: The study of mesenchymal stem cells, whether isolated from embryos or adults, provides the basis for the emergence of a new therapeutic technology of self‐cell repair.
Journal ArticleDOI
Marrow Stromal Cells as Stem Cells for Nonhematopoietic Tissues
TL;DR: Marrow stromal cells present an intriguing model for examining the differentiation of stem cells and have several characteristics that make them potentially useful for cell and gene therapy.
Journal ArticleDOI
In vitro chondrogenesis of bone marrow-derived mesenchymal progenitor cells
TL;DR: A culture system that facilitates the chondrogenic differentiation of rabbit bone marrow-derived mesenchymal progenitor cells has been developed in this article, where cells obtained in bone marrow aspirates were first isolated by monolayer culture and then transferred into tubes and allowed to form three-dimensional aggregates in a chemically defined medium.