Journal ArticleDOI
Hydrodynamic properties of complex, rigid, biological macromolecules: theory and applications.
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TLDR
In this paper, the translational and rotational brownian dynamics of dissolved macromolecules were analyzed using quasielastic light scattering, transient electric birefringence and fluorescence anisotropy decay.Abstract:
Among the Various methods for characterizing macromolecules in solution, hydrodynamic techniques play a major role. Since the advent of the ultracentrifuge and the development of viscometric apparatus, sedimentation coefficients and intrinsic viscosities have been extensively used to learn about the size and shape of synthetic and biological polymers. More recently, refined techniques such as quasielastic light scattering, transient electric birefringence and fluorescence anisotropy decay have made it possible to obtain in a simple and rapid way quantitative information of high precision on the translational and rotational brownian dynamics of dissolved macromolecules.read more
Citations
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Journal ArticleDOI
The Rotary Motor of Bacterial Flagella
TL;DR: Flagellated bacteria, such as Escherichia coli, swim by rotating thin helical filaments, each driven at its base by a reversible rotary motor, powered by an ion flux.
Journal ArticleDOI
Calculation of hydrodynamic properties of globular proteins from their atomic-level structure.
TL;DR: The solution properties, including hydrodynamic quantities and the radius of gyration, of globular proteins are calculated from their detailed, atomic-level structure, using bead-modeling methodologies described in the previous article, using a HYDROPRO public-domain computer program.
Journal ArticleDOI
Electrostatics and diffusion of molecules in solution: simulations with the University of Houston Brownian dynamics program
Jeffry D. Madura,James M. Briggs,Rebecca C. Wade,Malcolm E. Davis,Brock A. Luty,Andrew Ilin,J. Antosiewicz,Michael K. Gilson,Babak Bagheri,L. Ridgway Scott,J. Andrew McCammon +10 more
TL;DR: A general-purpose Brownian dynamics program that has been developed at the University of Houston is described in this paper, where the diffusion of flexible chains is modeled by the finite difference solutions of the linearized Poisson-Boltzmann equation.
Journal ArticleDOI
Backbone dynamics of calmodulin studied by 15N relaxation using inverse detected two-dimensional NMR spectroscopy: the central helix is flexible.
TL;DR: The backbone dynamics of Ca(2+)-saturated recombinant Drosophila calmodulin has been studied by 15N longitudinal and transverse relaxation experiments, combined with 15N(1H) NOE measurements, showing a high degree of mobility near the middle of the central helix and anisotropy observed in the motion of the two globular cal modulin domains is much smaller than expected.
Journal ArticleDOI
On the nucleation and growth of amyloid beta-protein fibrils: detection of nuclei and quantitation of rate constants
TL;DR: The approach provides a powerful means for the quantitative assay of A beta fibrillogenesis and found that the surfactant n-dodecylhexaoxyethylene glycol monoether (C12E6) slowed nucleation and elongation of fibrils in a concentration-dependent manner.
References
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Book
Low Reynolds number hydrodynamics
John Happel,Howard Brenner +1 more
TL;DR: Low Reynolds number flow theory finds wide application in such diverse fields as sedimentation, fluidization, particle-size classification, dust and mist collection, filtration, centrifugation, polymer and suspension rheology, and a host of other disciplines.
Journal ArticleDOI
A Theory of the Linear Viscoelastic Properties of Dilute Solutions of Coiling Polymers
TL;DR: In this paper, the necessary coordination of the motions of different parts of a polymer molecule is made the basis of a theory of the linear viscoelastic properties of dilute solutions of coiling polymers.
Journal ArticleDOI
Dynamics of Polymer Molecules in Dilute Solution: Viscoelasticity, Flow Birefringence and Dielectric Loss
TL;DR: In this paper, the authors considered the problem of diffusing a chain molecule diffusing in a viscous fluid under the influence of external forces or currents, and calculated the viscosity, birefringence of flow, and dielectric and tensile relaxation behavior explicitly.