Journal ArticleDOI
Hydrolysis of plant cuticle by plant pathogens. Properties of cutinase I, cutinase II, and a nonspecific esterase isolated from Fusarium solani pisi.
Purdy Re,Kolattukudy Pe +1 more
Reads0
Chats0
TLDR
The properties of the homogeneous cutinase I, cut inase II, and the nonspecific esterase isolated from the extracellular fluid of cutin-grown Fusarium solani F. pisi were investigated and the two cutinases showed similar substrate concentration dependent, protein concentration dependence, time course profiles, and pH dependence profiles.Abstract:
The properties of the homogeneous cutinase I, cutinase II, and the nonspecific esterase isolated from the extracellular fluid of cutin-grown Fusarium solani F. pisi (R.E. Purdy and P.E. Kolattukudy (1975), Biochemistry, preceding paper in this issue) were investigated. Using tritiated apple cutin as substrate, the two cutinases showed similar substrate concentration dependence, protein concentration dependence, time course profiles, and pH dependence profiles with optimum near 10.0. Using unlabeled cutin, the rate of dihydroxyhexadecanoic acid release from apple fruit cutin by cutinase I was determined to be 4.4 mumol per min per mg. The cutinases hydrolyzed methyl hexadecanoate, cyclohexyl hexadecanoate, and to a much lesser extent hexadecyl hexadecanoate but not 9-hexadecanoyloxyheptadecane, cholesteryl hexadecanoate, or hexadecyl cinnamate. The extent of hydrolysis of these model substrates by cutinase I was at least three times that by cutinase II. The nonspecific esterase hydrolyzed all of the above esters except hexadecyl cinnamate, and did so to a much greater extent than did the cutinases. None of the enzymes hydrolyzed alpha- or beta-glucosides of p-nitrophenol. p-Nitrophenyl esters of fatty acids from C2 through C18 were used as substrates and V's and Kms were determined...read more
Citations
More filters
Journal ArticleDOI
Efficient Mechano-Enzymatic Hydrolysis of Polylactic Acid under Moist-Solid Conditions
Karine Auclair,Tomislav Friščić +1 more
TL;DR: In this paper , a mechano-enzymatic depolymerization of polylactic acid to lactic acid was achieved at 55 °C using Humicola insolens cutinase enzyme in moist-solid reaction mixtures.
HYDROLYSIS OF BUTTEROIL BY FIBER REACTOR: PART V. EFFECTS OF pH. IMMOBILIZED LIPASE USING A HOLLOW-
TL;DR: A 14-parameter rate expression is necessary to accurately model the overall release of free fatty acids as a continuous function of pH, initial substrate concentration, reactor space time, and time elapsed after immobilization of the lipase.
Book ChapterDOI
Structural and Mutational Analysis of Polyethylene Terephthalate–Hydrolyzing Enzyme, Cut190, Based on Three-Dimensional Docking Structure with Model Compounds of Polyethylene Terephthalate
Posted ContentDOI
Biochemical characterization and NMR study of a PET-hydrolyzing cutinase from<i>Fusarium solani pisi</i>
TL;DR: In this paper , the kinetics of a PET-hydrolyzing cutinase from Fusarium solani pisi (FsC) at different pH values, mapped the interaction between FsC and the PET analog BHET by using NMR spectroscopy, and monitored product release directly and in real time by using time-resolved NMR experiments.
References
More filters
Journal ArticleDOI
A simple method for the isolation and purification of total lipides from animal tissues.
TL;DR: In this paper, the authors described a simplified version of the method and reported the results of a study of its application to different tissues, including the efficiency of the washing procedure in terms of the removal from tissue lipides of some non-lipide substances of special biochemical interest.
Journal ArticleDOI
The reliability of molecular weight determinations by dodecyl sulfate-polyacrylamide gel electrophoresis
Klaus Weber,Mary Osborn +1 more
TL;DR: The results show that the polyacrylamide gel electrophoresis method can be used with great confidence to determine the molecular weights of polypeptide chains for a wide variety of proteins.
Journal ArticleDOI
Determination of the structures of cutin monomers by a novel depolymerization procedure and combined gas chromatography and mass spectrometry.
T. J. Walton,P. E. Kolattukudy +1 more
Book ChapterDOI
3 Carboxylic Ester Hydrolases
TL;DR: This chapter focuses on B-esterases, which are inhibited stoichiometrically by organophosphates without hydrolyzing them, which have been formerly known as “ali-esterase” or, because of their wide specificity, as unspecific esterases.
Related Papers (5)
Hydrolysis of plant cuticle by plant pathogens. Purification, amino acid composition, and molecular weight of two isozymes of cutinase and a nonspecific esterase from Fusarium solani f. pisi.
Purdy Re,Kolattukudy Pe +1 more
Determination of the structures of cutin monomers by a novel depolymerization procedure and combined gas chromatography and mass spectrometry.
T. J. Walton,P. E. Kolattukudy +1 more