Journal ArticleDOI
Hydrolysis of plant cuticle by plant pathogens. Properties of cutinase I, cutinase II, and a nonspecific esterase isolated from Fusarium solani pisi.
Purdy Re,Kolattukudy Pe +1 more
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TLDR
The properties of the homogeneous cutinase I, cut inase II, and the nonspecific esterase isolated from the extracellular fluid of cutin-grown Fusarium solani F. pisi were investigated and the two cutinases showed similar substrate concentration dependent, protein concentration dependence, time course profiles, and pH dependence profiles.Abstract:
The properties of the homogeneous cutinase I, cutinase II, and the nonspecific esterase isolated from the extracellular fluid of cutin-grown Fusarium solani F. pisi (R.E. Purdy and P.E. Kolattukudy (1975), Biochemistry, preceding paper in this issue) were investigated. Using tritiated apple cutin as substrate, the two cutinases showed similar substrate concentration dependence, protein concentration dependence, time course profiles, and pH dependence profiles with optimum near 10.0. Using unlabeled cutin, the rate of dihydroxyhexadecanoic acid release from apple fruit cutin by cutinase I was determined to be 4.4 mumol per min per mg. The cutinases hydrolyzed methyl hexadecanoate, cyclohexyl hexadecanoate, and to a much lesser extent hexadecyl hexadecanoate but not 9-hexadecanoyloxyheptadecane, cholesteryl hexadecanoate, or hexadecyl cinnamate. The extent of hydrolysis of these model substrates by cutinase I was at least three times that by cutinase II. The nonspecific esterase hydrolyzed all of the above esters except hexadecyl cinnamate, and did so to a much greater extent than did the cutinases. None of the enzymes hydrolyzed alpha- or beta-glucosides of p-nitrophenol. p-Nitrophenyl esters of fatty acids from C2 through C18 were used as substrates and V's and Kms were determined...read more
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Journal ArticleDOI
Adhesion Pad Formation and the Involvement of Cutinase and Esterases in the Attachment of Uredospores to the Host Cuticle.
TL;DR: Whereas pad formation appears to be a passive response to the aqueous environment, the actual adhesion of pads to the host cuticle appears to depend on the cutinase and esterases associated with the spore surface, suggesting a new role for cutinases and serine-esterases in the fungal infection process.
Journal ArticleDOI
Disruption of a Magnaporthe Grisea Cutinase Gene
TL;DR: It is concluded that the M. grisea CUT1 gene is not required for pathogenicity and that the infection efficiency of the cut1− disruption transformants was equal to that of the parent strains on all three host plants.
Journal ArticleDOI
Cutinase Gene Disruption in Fusarium solani f sp pisi Decreases Its Virulence on Pea
TL;DR: Cutinase gene disruption resulted in a significant decrease in the pathogenicity of F. s.
Journal ArticleDOI
Structural and functional studies of Aspergillus oryzae cutinase: enhanced thermostability and hydrolytic activity of synthetic ester and polyester degradation.
Zhiqiang Liu,Yuying Gosser,P. J. Baker,Yaniv Ravee,Ziying Lu,Girum Alemu,Huiguang Li,Glenn L. Butterfoss,Xiang-Peng Kong,Richard A. Gross,Jin Kim Montclare +10 more
TL;DR: The reactivity, stability, and structure of Aspergillus oryzae cutinase is explored and it is compared to the well-studied enzyme from Fusarium solani to result in an improved hydrolytic activity and altered substrate specificity profile, enhanced thermostability, and remarkable reactivity toward the degradation of the synthetic polyester polycaprolactone.
References
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Journal ArticleDOI
A simple method for the isolation and purification of total lipides from animal tissues.
TL;DR: In this paper, the authors described a simplified version of the method and reported the results of a study of its application to different tissues, including the efficiency of the washing procedure in terms of the removal from tissue lipides of some non-lipide substances of special biochemical interest.
Journal ArticleDOI
The reliability of molecular weight determinations by dodecyl sulfate-polyacrylamide gel electrophoresis
Klaus Weber,Mary Osborn +1 more
TL;DR: The results show that the polyacrylamide gel electrophoresis method can be used with great confidence to determine the molecular weights of polypeptide chains for a wide variety of proteins.
Journal ArticleDOI
Determination of the structures of cutin monomers by a novel depolymerization procedure and combined gas chromatography and mass spectrometry.
T. J. Walton,P. E. Kolattukudy +1 more
Book ChapterDOI
3 Carboxylic Ester Hydrolases
TL;DR: This chapter focuses on B-esterases, which are inhibited stoichiometrically by organophosphates without hydrolyzing them, which have been formerly known as “ali-esterase” or, because of their wide specificity, as unspecific esterases.
Related Papers (5)
Hydrolysis of plant cuticle by plant pathogens. Purification, amino acid composition, and molecular weight of two isozymes of cutinase and a nonspecific esterase from Fusarium solani f. pisi.
Purdy Re,Kolattukudy Pe +1 more
Determination of the structures of cutin monomers by a novel depolymerization procedure and combined gas chromatography and mass spectrometry.
T. J. Walton,P. E. Kolattukudy +1 more