Journal ArticleDOI
Identification of a STS marker linked to the Aegilops speltoides-derived leaf rust resistance gene Lr28 in wheat
Suresh Naik,Kulvinder S. Gill,V. S. Prakasa Rao,Vidya S. Gupta,Shubhada Tamhankar,S. Pujar,Bikram S. Gill,Prabhakar K. Ranjekar +7 more
TLDR
A sequence-tagged-site (STS) marker is reported linked to Lr28, a leaf rust resistance gene in wheat, which was consistently present in the NILs, theresistant F3 bulk and the resistant F3 lines, but was absent in recurrent parents, the susceptible F3 Bulk and the susceptibleF3 lines.Abstract:
A sequence-tagged-site (STS) marker is reported linked to Lr28, a leaf rust resistance gene in wheat. RAPD (random amplified polymorphic DNA) analysis of near-isogenic lines (NILs) of Lr28 in eight varietal backgrounds was carried out using random primers. Genomic DNA enriched for low-copy sequences was used for RAPD analysis to overcome the lack of reproducibility due to the highly repetitive DNA sequences present in wheat. Of 80 random primers tested on the enriched DNA, one RAPD marker distinguished the NILs and the donor parent from the susceptible recurrent parents. The additional band present in resistant lines was cloned, sequenced, and STS primers specific for Lr28 were designed. The STS marker (Indian patent pending: 380 Del98) was further confirmed by bulk segregation analysis of F3 families. It was consistently present in the NILs, the resistant F3 bulk and the resistant F3 lines, but was absent in recurrent parents, the susceptible F3 bulk and the susceptible F3 lines.read more
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Journal ArticleDOI
Molecular markers and their applications in wheat breeding.
TL;DR: This review attempts to give an account of different molecular markers currently available for genome mapping and for tagging different traits in wheat, including restriction fragment length polymorphisms (RFLPs), random amplified polymorphic DNAs (RAPDs), sequence-tagged sites (STS), DNA amplification fingerprinting (DAF), amplified fragment length SNP (AFLPs) and microsatellites (STMS).
Journal ArticleDOI
An overview of molecular marker methods for plants
TL;DR: This article provides detail review for 11 different molecular marker methods: restriction fragment length polymorphism (RFLP), random amplified polymorphic DNA (RAPD), amplified fragment length polypeptide (AFLP%), inter-simple sequence repeats (ISSRs), sequence characterized regions (SCARs, sequence tag sites (STSs), cleaved amplified polymorphIC sequences (CAPS), microsatellites or simple sequence repeat (SSRs), expressed sequence tags (ESTs).
Journal ArticleDOI
Wheat Genomics: Present Status and Future Prospects
TL;DR: The availability of extensive cytogenetics stocks has been an asset, which facilitated significant progress in wheat genomic research in recent years as discussed by the authors, which can be used for future genomics research in this valuable crop.
Journal ArticleDOI
Utilisation of Aegilops (goatgrass) species to widen the genetic diversity of cultivated wheat
TL;DR: A large number of genes were transferred from Aegilops species to cultivated wheat, including those for resistance to leaf rust, stem rust, yellow rust and powdery mildew, and various pests.
Book ChapterDOI
The molecularization of public sector crop breeding: progress, problems, and prospects
Sangam L. Dwivedi,Jonathan H. Crouch,David J. Mackill,Yunbi Xu,Matthew W. Blair,Michel Ragot,Hari D. Upadhyaya,Rodomiro Ortiz +7 more
TL;DR: Advances in "omics" technologies are now assisting researchers to address complex biological issues of significant agricultural importance: modeling genotype-by-environment interaction; fine-mapping, cloning, and pyramiding of QTL; gene expression analysis and gene function elucidation; dissecting the genetic structure of germplasm collections to mine novel alleles and develop genetically structured trait-based core collections; and understanding the molecular basis of heterosis.
References
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Molecular Cloning: A Laboratory Manual
TL;DR: Molecular Cloning has served as the foundation of technical expertise in labs worldwide for 30 years as mentioned in this paper and has been so popular, or so influential, that no other manual has been more widely used and influential.
Journal ArticleDOI
DNA sequencing with chain-terminating inhibitors
TL;DR: A new method for determining nucleotide sequences in DNA is described, which makes use of the 2',3'-dideoxy and arabinon nucleoside analogues of the normal deoxynucleoside triphosphates, which act as specific chain-terminating inhibitors of DNA polymerase.
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RFLP Mapping in Plant Breeding: New Tools for an Old Science
TL;DR: RFLP linkage maps provide a more direct method for selecting desirable genes via their linkage to easily detectable RFLP markers and may make it possible to clone genes whose products are unknown, such as genes for disease resistance or stress tolerance.
Book ChapterDOI
Extraction of DNA from plant tissues
TL;DR: Extraction procedures for plant DNA in general must accomplish the following: break the cell walls, protect the DNA from the endogenous nucleases, and minimize the time between thawing of the frozen, pulverized tissue and its exposure to the extraction buffer.
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Rust diseases of wheat: Concepts and methods of disease management
TL;DR: A partir de las obras de Tozzeni y Fontana en 1767, existe una lista muy extensa de publicaciones cientificas sobre los agentes patogenos of las royas, las enfections that provocan and the resistencia a ellas as mentioned in this paper.