Identification of MAD2L1 as a Potential Biomarker in Hepatocellular Carcinoma via Comprehensive Bioinformatics Analysis
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TLDR
It is found that high MAD2L1 expression in HCC was significantly associated with overall patient survival and clinical features and the potential biological properties of this gene were explored.Abstract:
Background Hepatocellular carcinoma (HCC) is widely acknowledged as a malignant tumor with rapid progression, high recurrence rate, and poor prognosis. At present, there is a paucity of reliable biomarkers at the clinical level to guide the management of HCC and improve patient outcomes. Our research is aimed at assessing the prognostic value of MAD2L1 in HCC. Methods Four datasets, GSE121248, GSE101685, GSE85598, and GSE62232, were selected from the GEO database to analyze differentially expressed genes (DEGs) between HCC and normal liver tissues. After functional analysis, we constructed a protein-protein interaction network (PPI) for DEGs and identified core genes in this network with high connectivity with other genes. We assessed the relationship between core genes and the pathogenesis and prognosis of HCC. Finally, we explored the gene regulatory signaling mechanisms involved in HCC pathogenesis. Results 145 DEGs were screened from the intersection of the four GEO datasets. MAD2L1 was associated with most genes according to the PPI network and was selected as a candidate gene for further study. Survival analysis suggested that high MAD2L1 expression in HCC correlated with a worse prognosis. In addition, real-time quantitative PCR (RT-qPCR), western blot (WB), and immunohistochemistry (IHC) findings suggested that the expression of MAD2L1 was abnormally increased in HCC tissues and cells compared to paraneoplastic tissues and normal hepatocytes. Conclusion We found that high MAD2L1 expression in HCC was significantly associated with overall patient survival and clinical features. We also explored the potential biological properties of this gene.read more
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References
More filters
Journal ArticleDOI
Cancer Statistics, 2021.
TL;DR: In the United States, the cancer death rate has dropped continuously from its peak in 1991 through 2018, for a total decline of 31%, because of reductions in smoking and improvements in early detection and treatment as mentioned in this paper.
Journal ArticleDOI
The STRING database in 2021: customizable protein-protein networks, and functional characterization of user-uploaded gene/measurement sets.
Damian Szklarczyk,Annika L. Gable,Katerina C. Nastou,David Lyon,Rebecca Kirsch,Sampo Pyysalo,Nadezhda Tsankova Doncheva,Marc Legeay,Tao Fang,Peer Bork,Lars Juhl Jensen,Christian von Mering +11 more
TL;DR: Changes to the text-mining system, a new scoring-mode for physical interactions, as well as extensive user interface features for customizing, extending and sharing protein networks are described.
Journal ArticleDOI
Cancer statistics for the year 2020: An overview
Jacques Ferlay,Murielle Colombet,Isabelle Soerjomataram,Donald Maxwell Parkin,Donald Maxwell Parkin,Marion Piñeros,Ariana Znaor,Freddie Bray +7 more
TL;DR: This paper reviewed the data sources and methods used in compiling the International Agency for Research on Cancer (IARC) GLOBOCAN cancer statistics for the year 2020 and summarised the main results.
Journal ArticleDOI
Radiofrequency ablation for hepatocellular carcinoma in so-called high-risk locations.
Takuma Teratani,Haruhiko Yoshida,Shuichiro Shiina,Shuntaro Obi,Shinpei Sato,Ryosuke Tateishi,Norio Mine,Yuji Kondo,Takao Kawabe,Masao Omata +9 more
TL;DR: In conclusion, HCC nodules adjacent to a large vessel or extrahepatic organ were treated with RF ablation without compromising the efficacy of the procedure, however, even though without significant difference, some complications occurred at risky locations and need to be carefully considered.
Book ChapterDOI
Hepatocellular carcinoma (HCC): Epidemiology, etiology and molecular classification.
TL;DR: This review examines the results of efforts in classifying HCC at the molecular, metabolic and immunologic levels and the ways they can be leveraged to develop targeted treatment options for HCC.