Journal ArticleDOI
Image reconstruction of the subbasal nerve plexus with in vivo confocal microscopy.
Stephan Allgeier,Andrey Zhivov,F. Eberle,Bernd Koehler,S. Maier,Georg Bretthauer,Rudolf F. Guthoff,Oliver Stachs +7 more
TLDR
The described method allows the precise analysis and elimination of motion artifacts in CLSM volume scans, in conjunction with the capability to reconstruct SNP structures even in the presence of severe ACM, providing a sound basis for extended studies of corneal nerve regeneration or degeneration.Abstract:
PURPOSE To overcome the anterior corneal mosaic (ACM) phenomenon in in vivo confocal laser scanning microscopy (CLSM) and to reconstruct undistorted images of the subbasal nerve plexus (SNP), facilitating morphometric analysis in the presence of ACM ridges METHODS CLSM was performed in five healthy volunteers An original image processing algorithm based on phase correlation was used to analyze and reduce motion distortions in volume scan image sequences Three-dimensional tracing of the SNP was performed to reconstruct images containing only the SNP layer, with nerve fibers clearly visible even in ACM areas RESULTS Real-time mapping of the SNP revealed the presence of ridges with K-structures underneath them in all cases The occurrence of K-structures correlated directly with development of ACM observed by slit lamp and resulted in massive deformation at the level of Bowman’s membrane, seriously interfering with examination of SNP structures The average elevation of ACM ridges was 206 m (range, 87‐340 m) The novel method presented permitted reconstruction of the SNP layer in regions of ACM CONCLUSIONS The described method allows the precise analysis and elimination of motion artifacts in CLSM volume scans, in conjunction with the capability to reconstruct SNP structures even in the presence of severe ACM The robustness and automation of the described algorithms require ongoing development, but this will provide a sound basis for extended studies of corneal nerve regeneration or degeneration and for use in clinical practice (Invest Ophthalmol Vis Sci 2011;52: 5022‐5028) DOI:101167/iovs10-6065read more
Citations
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Journal ArticleDOI
Early Detection of Nerve Fiber Loss by Corneal Confocal Microscopy and Skin Biopsy in Recently Diagnosed Type 2 Diabetes
Dan Ziegler,Nikolaos Papanas,Andrey Zhivov,Stephan Allgeier,Karsten Winter,Iris Ziegler,Jutta Brüggemann,Alexander Strom,Sabine Peschel,Bernd Köhler,Oliver Stachs,Rudolf F. Guthoff,Michael Roden +12 more
TL;DR: CCM and skin biopsy both detect nerve fiber loss in recently diagnosed type 2 diabetes, but largely in different patients, suggesting a patchy manifestation pattern of small fiber neuropathy.
Journal ArticleDOI
In Vivo Confocal Microscopy of Corneal Nerves in Health and Disease
TL;DR: The principles and applications of IVCM are outlined, which are increasingly important in the diagnosis and management of systemic conditions such as peripheral diabetic neuropathy and, more recently, in ocular diseases, and in the study of corneal nerves in various ocular and systemic diseases.
Journal ArticleDOI
Corneal nerve structure and function in patients with non-sjogren dry eye: clinical correlations.
Antoine Labbé,Qingfeng Liang,Zhiqun Wang,Yang Zhang,Liang Xu,Christophe Baudouin,Xuguang Sun +6 more
TL;DR: NSDD patients have both structural and functional alterations of subbasal corneal nerves and these changes are related to the severity of dry eye.
Journal ArticleDOI
The Corneal Pain System. Part I: The Missing Piece of the Dry Eye Puzzle
Perry Rosenthal,David Borsook +1 more
TL;DR: This enigmatic disease can be viewed with a new perspective, which involves the dysfunctional corneal pain system as a central pathogenetic feature of a series of disorders collectively known today as dry eye.
Journal ArticleDOI
In vivo confocal microscopy of the ocular surface: from bench to bedside.
Edoardo Villani,Christophe Baudouin,Nathan Efron,Pedram Hamrah,Takashi Kojima,Sanjay V. Patel,Stephen C. Pflugfelder,Andrey Zhivov,Murat Dogru +8 more
TL;DR: In vivo confocal microscopy is particularly useful in the study of corneal nerves, enabling description of the morphology, density, and disease- or surgically induced alterations of nerves, particularly the subbasal nerve plexus.
References
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The role of fixational eye movements in visual perception
TL;DR: Current studies of fixational eye movements have focused on determining how visible perception is encoded by neurons in various visual areas of the brain to elucidate how the brain makes the authors' environment visible.
Journal ArticleDOI
Surrogate Markers of Small Fiber Damage in Human Diabetic Neuropathy
Cristian Quattrini,Mitra Tavakoli,Maria Jeziorska,Panagiotis A. Kallinikos,Solomon Tesfaye,Joanne Finnigan,Andrew Marshall,Andrew J.M. Boulton,Nathan Efron,Rayaz A. Malik +9 more
TL;DR: In this paper, the authors have quantified small nerve fiber pathological changes using the technique of intraepidermal nerve fiber (IENF) assessment and the novel in vivo technique of corneal confocal microscopy (CCM).
Journal ArticleDOI
Corneal confocal microscopy: a non-invasive surrogate of nerve fibre damage and repair in diabetic patients
Rayaz A. Malik,Panagiotis A. Kallinikos,Caroline A. Abbott,C. H. M. van Schie,Philip B. Morgan,Nathan Efron,Andrew J.M. Boulton +6 more
TL;DR: In this article, the ability of confocal microscopy to quantify the extent of degeneration and regeneration of corneal nerve fibres in diabetic patients with increasing neuropathic severity was defined.
Journal Article
Surrogate markers of small fiber damage in human diabetic neuropathy
Cristian Quattrini,Mitra Tavakoli,Maria Jeziorska,Panagiotis A. Kallinikos,Solomon Tesfaye,Joanne Finnigan,Andrew Marshall,Andrew J.M. Boulton,Nathan Efron,Rayaz A. Malik +9 more
TL;DR: Intraepidermal and corneal nerve fiber lengths were reduced in patients with painful compared with painless diabetic neuropathy, and both IENF and CCM assessment accurately quantify small nerve fiber damage in diabetic patients.
Corneal confocal microscopy: a non-invasive surrogate of nerve fibre damage and repair in diabetic patients
Rayaz A. Malik,Panagiotis A. Kallinikos,Caroline A. Abbott,Carine H.M. van Schie,Philip B. Morgan,Nathan Efron,Andrew J.M. Boulton +6 more
TL;DR: In this article, the authors defined the ability of this technique to quantify the extent of degeneration and regeneration of corneal nerve fibres in diabetic patients with increasing neuropathic severity.