Journal ArticleDOI
Imaging mass spectrometry: a new technology for the analysis of protein expression in mammalian tissues.
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Imaging MS joins techniques such as immunochemistry and fluorescence microscopy for the study of the spatial arrangement of molecules within biological tissues for the unraveling and understanding the molecular complexities of cells.Abstract:
1and has been initially targeted for the analysis of peptides and proteins present on or near the surface of tissue sections 2 . Imaging MS brings a new tool to bear on the problem of unraveling and understanding the molecular complexities of cells. It joins techniques such as immunochemistry and fluorescence microscopy for the study of the spatial arrangement of molecules within biological tissues. Many previous experiments using MS to image samples have focused on the measurement of the distribution of elements and small molecules in biological specimens, including tissue slices and individual cells 3‐5 . An extensive review on imaging by MS can be found in the article by Pacholski and Winograd 6read more
Citations
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Journal ArticleDOI
Mass spectrometry-based proteomics
Ruedi Aebersold,Matthias Mann +1 more
TL;DR: The ability of mass spectrometry to identify and, increasingly, to precisely quantify thousands of proteins from complex samples can be expected to impact broadly on biology and medicine.
Journal ArticleDOI
Mass Spectrometry Sampling Under Ambient Conditions with Desorption Electrospray Ionization
TL;DR: The DESI phenomenon was observed both in the case of conductive and insulator surfaces and for compounds ranging from nonpolar small molecules such as lycopene, the alkaloid coniceine, and small drugs, through polar compounds such as peptides and proteins.
Journal ArticleDOI
Imaging mass spectrometry
TL;DR: Many of the instrumental developments and methodological approaches responsible for an increasing upsurge in interest in imaging mass spectrometry are reviewed, compare and contrast the information provided by SIMS and MALDI imaging, and discuss future possibilities.
Journal ArticleDOI
Proteomic applications for the early detection of cancer.
TL;DR: Proteomic analyses of early-stage cancers have provided new insights into the changes that occur in the early phases of tumorigenesis and represent a new resource of candidate biomarkers for early stage disease as discussed by the authors.
Journal ArticleDOI
Ambient mass spectrometry using desorption electrospray ionization (DESI): instrumentation, mechanisms and applications in forensics, chemistry, and biology
TL;DR: Examples of applications to small and large molecules, to in situ analysis, and to high-throughput analyses are presented and evidence is provided for both a heterogeneous charge-transfer mechanism and a droplet pick-up mechanism of ionization.
References
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Journal ArticleDOI
Proposal for a common nomenclature for sequence ions in mass spectra of peptides.
Roepstorff P,Fohlman J +1 more
Journal ArticleDOI
Molecular Imaging of Biological Samples: Localization of Peptides and Proteins Using MALDI-TOF MS
TL;DR: Matrix-assisted laser desorption/ionization mass spectrometry (MALDI MS) has been used to generate ion images of samples in one or more mass-to-charge (m/z) values, providing the capability of mapping specific molecules to two-dimensional coordinates of the original sample.
Journal ArticleDOI
Matrix-assisted ultraviolet laser desorption of non-volatile compounds
TL;DR: In this paper, the use of a matrix for pulsed ultraviolet laser desorption mass spectrometry is shown to extend its applicability into the range of larger, thermally labile biomolecules.
Journal ArticleDOI
Isolation and characterization of human malignant glioma cells from histologically normal brain
TL;DR: Tumor cells were cultured from histologically normal brain acquired from a distance greater than 4 cm from the gross tumor, indicating the relative insensitivity of standard histopathological identification of invasive glioma cells (and hence the inadequacy of frozen-section evaluation of resection margins).
Journal ArticleDOI
Direct profiling of proteins in biological tissue sections by MALDI mass spectrometry.
TL;DR: The direct profiling of proteins present in tissue sections for several organs of the mouse has been accomplished using matrix-assisted laser desorption ionization (MALDI) mass spectrometry (MS), with unique protein profiles measured from these tissue blots.