Journal ArticleDOI
Improved Methods for Cobalt Filling and Silver Intensification of Insect Motor Neurons
TLDR
A Parafilm disk floating on saline and bearing a drop of cobalt solution in which the nerve stump is bathed provides a convenient and versatile method for in vitro or in vivo filling of neurons for cobalt sulfide staining.Abstract:
A Parafilm disk floating on saline and bearing a drop of cobalt solution in which the nerve stump is bathed provides a convenient and versatile method for in vitro or in vivo filling of neurons for cobalt sulfide staining. Silicone grease around the edge of the disk provides an effective seal around the nerve as it passes between the two solutions.Using a modified developer, silver intensification of cobalt sulfide stained neurons may be done in the light at room temperature, and the time of optimum intensification may be observed under a dissecting microscope.read more
Citations
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Journal ArticleDOI
Visualization of O-2A progenitor cells in developing and adult rat optic nerve by quisqualate-stimulated cobalt uptake
TL;DR: The results suggest that the cells stimulated by quisqualate to take up cobalt in the optic nerve are the in vivo counterpart of O-2A progenitor cells, and no evidence that any of these cells are type 2 astrocytes.
Journal ArticleDOI
Intracellular lucifer yellow injection in fixed brain slices combined with retrograde tracing, light and electron microscopy.
E.H. Buhl,Joachim H. R. Lübke +1 more
TL;DR: Intracellular staining in fixed tissue yields a high number of neurons with extensive filling of dendritic arbors, rendering the method to be a promising alternative to classical neuroanatomical approaches.
Journal ArticleDOI
The distribution of neurons expressing calcium-permeable AMPA receptors in the superficial laminae of the spinal cord dorsal horn.
TL;DR: It is proposed that Ca2+-permeable AMPA receptors are localized to mediate transmission of nociceptive information in the postnatal rat dorsal horn.
Journal ArticleDOI
Identification of the neuropeptide transmitter proctolin in Drosophila larvae: characterization of muscle fiber-specific neuromuscular endings
TL;DR: The cellular localization of the peptide neurotransmitter proctolin was determined for larvae of the fruitfly Drosophila melanogaster and it is proposed that a specialized subset of muscle fibers are also innervated by peptidergic efferents.
Journal ArticleDOI
Crustacean cardioactive peptide-immunoreactive neurons in the hawkmoth Manduca sexta and changes in their immunoreactivity during postembryonic development
TL;DR: Many of the neurons identified here are comparable to crustacean cardioactive peptide‐immunoreactive cells described previously in locusts and the mealworm beetle.
References
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Journal ArticleDOI
A silver intensification method for cobalt-filled neurones in wholemount preparations.
J.P. Bacon,J.S. Altman +1 more
TL;DR: A simple variant of the Timm's sulphide-silver intensification method for whole ganglia is reported here on, and trials are conducted to determine the optimum conditions for the intensification process.
Journal ArticleDOI
Branching of Central Neurons: Intracellular Cobalt Injection for Light and Electron Microscopy
TL;DR: In whole mount preparations, the resolution of neurites within the neuropil is of a quality that permits the comparison of branching patterns between cells and during various functional states.
Journal ArticleDOI
[Histochemistry of heavy metals; the sulfide-silver procedure].
TL;DR: Die Gelegenheit, nach so vielen Jahren wieder an unserer Tagung teilnehmen zu kSnnen, will ich benutzen, Ihnen fiber Untersuchungsergebnisse aus den Jahren 1938--1945 in Leipzig and Jena zu berichten.
Journal ArticleDOI
The intensification of cobalt-filled neurone profiles using a modification of Timm's sulphide-silver method.
N.M. Tyrer,E.M. Bell +1 more
Journal ArticleDOI
The morphology of motoneurons and dorsal root fibers in the frog's spinal cord
TL;DR: Ventral and dorsal roots of the frog's spinal cord were filled with cobaltous chloride, and the resulting Cobaltous sulfide precipitate, following treatment with H2S-buffer solutions, was intensified with physical developers to distinguish a ventromedial and a dorsolateral motoneuron group.
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