Journal ArticleDOI
Improvement of Riboflavin Production by Lactobacillus fermentum Isolated from Yogurt
TLDR
The riboflavin production by Lactobacillus fermentum isolated from yogurt was improved by inactivation of folE gene, which codes for GTP cyclohydrolase I, which serves as the precursor for both folate as well as rib oflavin biosynthesis.Abstract:
The riboflavin production by Lactobacillus fermentum isolated from yogurt was improved by inactivation of folE gene. Guanosine triphosphate (GTP) serves as the precursor for both folate as well as riboflavin biosynthesis. The folE gene of Lb. fermentum, which codes for GTP cyclohydrolase I was inactivated by the insertion of erythromycin resistance gene cassette through recombination and the riboflavin production by the parental and mutant strains were estimated. Inactivation of the folE gene involved in folate biosynthesis pathway resulted in enhanced level of riboflavin production by Lb. fermentum, since more amount of GTP molecules are made available for the GTP cyclohydrolase II mediated riboflavin biosynthesis pathway. In a chemically defined medium, the Lb. fermentum produced 2.29 mg L−1 of riboflavin at 24 h of fermentation. Whereas the folE disrupted mutant Lb. fermentum GKJFE produced approximately 50% more riboflavin (3.49 mg L−1) than that of the parental strain at 72 h of fermentation.read more
Citations
More filters
Journal Article
Research letterThe relation between growth rate and electrochemical proton gradient of Streptococcus cremoris
TL;DR: In this paper, the authors present the results of a study on the magnitude and composition of the electrochemical proton gradient (Ak~H+) of Streptococcus cremoris growing in continuous cultures at different rates and different pH values.
Journal ArticleDOI
Lactic acid bacteria as a cell factory for riboflavin production
TL;DR: The present review attempts to collect the currently available information on riboflavin production by microbes in general, while placing greater emphasis on food grade lactic acid bacteria and human gut commensals.
Journal ArticleDOI
Production of riboflavin and related cofactors by biotechnological processes.
TL;DR: The biotechnological processes for the production of RF and its cognate cofactors are reviewed in this article and it is shown that developing microbial cell factories that are capable of efficiently producing flavin nucleotides at low cost is an increasingly attractive approach.
Journal ArticleDOI
Relationships between the genome and some phenotypical properties of Lactobacillus fermentum CECT 5716, a probiotic strain isolated from human milk
Nivia Cárdenas,Jonathan Emiliano Laiño,Susana Delgado,Esther Jiménez,Marianela Juárez del Valle,Graciela Savoy de Giori,Fernando Sesma,Baltasar Mayo,Leónides Fernández,Jean Guy LeBlanc,Juan M. Rodríguez +10 more
TL;DR: Lactobacillus fermentum CECT 5716, isolated from human milk, has immunomodulatory, anti-inflammatory, and anti-infectious properties, as revealed by several in vitro and in vivo assays, which suggests a strong potential as a probiotic strain.
Journal ArticleDOI
Recent update on lactic acid bacteria producing riboflavin and folates: application for food fortification and treatment of intestinal inflammation.
TL;DR: The potential uses of riboflavin and folates producing LAB for the biofortification of food, as therapeutics against intestinal pathologies and to complement anti‐inflammatory/anti‐neoplastic treatments are shown.
References
More filters
Book
Molecular Cloning: A Laboratory Manual
TL;DR: Molecular Cloning has served as the foundation of technical expertise in labs worldwide for 30 years as mentioned in this paper and has been so popular, or so influential, that no other manual has been more widely used and influential.
Journal ArticleDOI
A medium for the cultivation of lactobacilli
TL;DR: An improved growth medium for lactobacilli is described, which supports good growth of lactOBacilli generally and also is particularly useful for a number of fastidious strains which grow only poorly in other general media.
Journal ArticleDOI
Biopreservation by lactic acid bacteria.
TL;DR: This paper reviews the current status and potential for controlled biopreservation of foods and lactic acid bacteria.
Journal ArticleDOI
Three biotechnical processes using Ashbya gossypii, Candida famata, or Bacillus subtilis compete with chemical riboflavin production.
TL;DR: The production of riboflavin by the two fungi seems to be limited by precursor supply, as was concluded from feeding and gene-overexpression experiments, and the major limitation there seems to to be the rib oflavin pathway.
Journal ArticleDOI
Incompatibility of Lactobacillus Vectors with Replicons Derived from Small Cryptic Lactobacillus Plasmids and Segregational Instability of the Introduced Vectors.
M. Posno,R.J. Leer,N. van Luijk,M. J. F. van Giezen,P. T. H. M. Heuvelmans,B.C. Lokman,P.H. Pouwels +6 more
TL;DR: Lactobacillus vectors are generally rapidly lost when cells are cultivated in the absence of selective pressure, however, pLPE323 is stable in three of four LactOBacillus strains tested so far and is most likely to be explained by the incompatibility of the vectors and resident plasmids.