Book ChapterDOI
In Situ Immobilization of Enzymes in Biomimetic Silica.
TLDR
Different approaches for the immobilization and co-immobilization of enzymes that could be potentially extensible to other biocatalysts are provided.Abstract:
In this chapter we describe different strategies for enzyme immobilization in biomimetic silica nanoparticles. Synthesis of this type of support is performed under mild and biocompatible conditions and has been proven suitable for the immobilization and stabilization of a range of enzymes and enzymatic systems in nanostructured particles. Immobilization occurs by entrapment while the silica matrix is formed via catalysis of a polyamine molecule and the presence of silicic acid. Parameters such as enzyme, polyamine molecule, or source of Si concentration have been tailored in order to maximize enzymatic loads, stabilities, and specific activities of the catalysts. We provide different approaches for the immobilization and co-immobilization of enzymes that could be potentially extensible to other biocatalysts.read more
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Journal ArticleDOI
Emerging opportunities of silica-based materials within the food industry
TL;DR: In this paper, the most common synthesis pathways to develop colloidal silicas and silica-metal hybrids are discussed, and a special emphasis regarding to their applications within the food sector is given, more specifically, as host/carriers for enzyme immobilization in food production and processing, as materials to produce sensor-like devices for food-related analyte detection in food safety monitoring, and as inorganic fillers in polymer composite formation.
Journal ArticleDOI
Stabilization of b-Glucuronidase by Immobilization in Magnetic-Silica Hybrid Supports
TL;DR: The hybrid material produced herein demonstrated its versatility and robustness as a support for β-Glucuronidases immobilization as well as storage, thermal, and operational stability were superior for the enzyme immobilized in the composite material.
Journal ArticleDOI
Stabilization of ω-transaminase from Pseudomonas fluorescens by immobilization techniques.
Susana Velasco-Lozano,Erienne Jackson,Magdalena Ripoll,Fernando López-Gallego,Fernando López-Gallego,Lorena Betancor +5 more
TL;DR: This is the first report describing a transaminase cross-linked enzyme aggregate as immobilization strategy to generate a biocatalyst with outstanding thermostability.
Journal ArticleDOI
Green Production of Cladribine by Using Immobilized 2'-Deoxyribosyltransferase from Lactobacillus delbrueckii Stabilized through a Double Covalent/Entrapment Technology.
Cintia W. Rivero,Natalia Soledad García,Jesús Fernández-Lucas,Lorena Betancor,Gustavo Pablo Romanelli,Jorge A. Trelles,Jorge A. Trelles +6 more
TL;DR: In this paper, a 2'-deoxyribosyltransferase (NDT)-based combined biocatalyst was used for the green production of cladribine at mg scale.
Journal ArticleDOI
Immobilization and stabilization of enzymes using biomimetic silicification reactions
TL;DR: This work reviews the literature of the last five years on biomimetic silica immobilized enzymes with a special focus on the stabilization gained by the biocatalysts and the rationale behind it.
References
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Journal ArticleDOI
Enzyme immobilization in a biomimetic silica support.
TL;DR: It is shown that butyrylcholinesterase entrapped during the precipitation of silica nanospheres retained all of its activity and was substantially more stable than the free enzyme.
Journal ArticleDOI
A general overview of support materials for enzyme immobilization: Characteristics, properties, practical utility
TL;DR: A general overview of the characteristics and properties of the materials applied for enzyme immobilization can be found in this article, where support materials are divided into two main groups, called Classic and New materials.
Journal ArticleDOI
Diatoms—From Cell Wall Biogenesis to Nanotechnology
Nils Kröger,Nicole Poulsen +1 more
TL;DR: Analysis of the organic components associated with diatom silica, the development of techniques for molecular genetic manipulation of diatoms, and two diatom genome sequencing projects are providing insight into the composition and mechanism of the remarkable biosilica-forming machinery.
Journal ArticleDOI
Bioinspired enzyme encapsulation for biocatalysis.
TL;DR: This review discusses biomimetic and bioinspired mineral formation as a technique for the immobilization of enzymes with potential application to a wealth of biocatalytic processes.
Journal ArticleDOI
Categorization of enzyme deactivations using a series-type mechanism
James P. Henley,Ajit Sadana +1 more
TL;DR: A series-type enzyme deactivation model involving an active enzyme precursor and a final enzyme state with possible non-zero activity is proposed to categorize enzyme de activation curves.