In-vitro cytotoxicity activity of solanum nigrum extract against hela cell line and vero cell line.

TLDR: From the performed assay, methanolic extract of these drug shows greater activity on HeLa cell line and little activity on Verocell line and that mean Solanum Nigrum can be used as anticancer.

Abstract: The study was aimed to evaluation of the anticancer activity of the fruits of Solanum Nigrum on the HeLa cell line. The fruits of Solanum Nigrum methanolic extract were tested for its inhibitory effect on HeLa Cell Line. The percentage viability of the cell line was carried out by using Trypan blue dye exclusion method. The cytotoxicity of Solanum Nigrum on HeLa cell was evaluated by the SRB assay and MTT assay. Solanum Nigrum methanolic extract has significant cytotoxicity effect on HeLa Cell Line in concentration range between 10 mg/ml to 0.0196 mg/ml by using SRB assay and study also showed that inhibitory action on HeLa cell line in concentration range between 10 mg/ml to 0.0196 mg/ml by using MTT assay. IC50 value and R 2 value of Solanum Nigrum on HeLa cell and Vero cell were 847.8 and 0.8724, 9088 and 0.1017 respectively by SRB assay. IC50 value and R 2 value of Solanum Nigrum on HeLa cell was 265.0 and 0.9496 respectively by MTT assay. IC50 value of Solanum Nigrum on Vero cell was 6.862 by MTT assay. R 2 value of Solanum Nigrum was not found by MTT assay. From the performed assay, methanolic extract of these drug shows greater activity on HeLa cell line and little activity on Vero cell line and that mean Solanum Nigrum can be used as anticancer