In vitro Quantification of Collagen and Snail1 Gene Expression in Experimentally Induced Fibrosis by Arecoline and Commercial Smokeless Tobacco Products.
TLDR
In this paper, an extensive quantification of the components with varying concentrations of areca nut extract and commercial smokeless tobacco products have been done, and the results showed increased collagen production.Abstract:
Introduction: Extracellular matrix component derangement is the major event in pathogenesis of Oral submucous fibrosis. Many studies have elaborated the alteration of the matrix components at a cellular and genetic level. However elaborate quantification of the components with varying concentrations of Areca nut extract and commercial tobacco products have not been done so far. Materials and Methods: Primary culture of tissues sourced during crown lengthening procedures were used for establishment of fibroblast monoculture and fibroblast / keratinocyte co-culture. Extracts of areca nut, commercial smokeless tobacco products (gutkha and haans) and control CCl4 were tested at concentrations ranging from 20 μL, 40 μL, 80 μL, 160 μL, 320 μL and time intervals of 12, 24, 48, 72 hours. Collagen quantification by spectrophotometry and SNAI1 gene expression study were done. Results: Extract of areca nut was found to show increased collagen production than commercial tobacco products and closely similar values to CCL4. Kruskal Wallis test was used to analyse the difference in collagen obtained. The mean values of collagen obtained in co-culture were lesser than those obtained in the fibroblast monoculture. SNAI1 gene expression was negative in both the culture experiments. Conclusion: Areca nut extract was found to be more potent as an individual agent. Commercial smokeless tobacco products Gutka and Hans exhibited increased collagen production at higher concentration. These findings further steps up the persuasive ill effects of tobacco products. Negative SNAI1 gene expression was corroborated to lack of extracellular environment in the co coculture experiment.read more
Citations
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Journal ArticleDOI
Genetic Susceptibility and Protein Expression of Extracellular Matrix Turnover-Related Genes in Oral Submucous Fibrosis.
Ru-Hsiu Cheng,Yi Ping Wang,Julia Yu Fong Chang,Yu-Hwa Pan,Mei-Chi Chang,Mei-Chi Chang,Jiiang-Huei Jeng +6 more
TL;DR: Betel quid chewing increased the risk of oral cancer and oral submucous fibrosis (OSMF), an oral premalignant disorder (OPMD) with malignant transformation potential and genetic susceptibility is also involved in these disease processes.
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Molecular Mechanisms of Malignant Transformation of Oral Submucous Fibrosis by Different Betel Quid Constituents—Does Fibroblast Senescence Play a Role?
Pangzhen Zhang,Nathaniel Quan En Chua,Simon Dang,Ashleigh M. Davis,Kah Wee Chong,Stephen S. Prime,Nicola Cirillo +6 more
TL;DR: The characterization of the detrimental or protective effects of specific BQ ingredients may facilitate the development of targeted BQ formulations to prevent and/or treat potentially malignant oral disorders and oral cancer in BQ users.
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Anti-fibrotic activity of licorice extract in comparison with colchicine on areca nut-induced fibroblasts: An in vitro study
A. H. James,Nandhini Devi Gunasekaran,Rajkumar Krishnan,Preethi Arunachalam,Ramya Mahalingam +4 more
TL;DR: The current study proves the antifibrotic efficacy of licorice in areca nut induced cell lines and hence, this agent can be used for the therapeutic management of OSMF.
Journal ArticleDOI
Interleukin‐13 contributes to the occurrence of oral submucosal fibrosis
TL;DR: In this paper , the role of interleukin (IL)13 in the development of oral submucous fibrosis (OSF) was investigated and further explored whether IL−13 regulates the polarization of M2-macrophages in OSF.
Proceedings ArticleDOI
Effect Of Various Tobacco Product On The PH And The Cytomorphology Of Blood:A Comparative Study
R Priyadharshini,P. Sinduja +1 more
TL;DR: In this article , the effects of different tobacco products on blood pH and cytomorphology were investigated, and it was shown that smokeless tobacco and bidi smoking increased blood pH (7.2 and 7.4, respectively), making the blood more alkaline, while filtered cigarettes decreased blood pH.
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TL;DR: Fibroblasts are responsive to the major metabolite of arecoline and hydrolysis of the ester group may be necessary for this action, which may contribute to the accumulation of collagen in OSF.
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