Journal ArticleDOI
Increase in chromatin associated nuclease activity of excised barley leaves during senescence and its suppression by kinetin
TLDR
No significant changes in the composition of chromatin were found when the excised barley leaves were floated on water or on kinetin solution in the dark, but increases in the activities ofchromatin associated ribonuclease and deoxyribonucleasing in leaves floated onWater drastically suppressed the increases in these enzymes.About:
This article is published in Biochemical and Biophysical Research Communications.The article was published on 1968-08-13. It has received 42 citations till now. The article focuses on the topics: Kinetin & Deoxyribonuclease.read more
Citations
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Journal ArticleDOI
Function of Lysosomes and Lysosomal Enzymes in the Senescing Corolla of the Morning Glory (Ipomoea purpurea)
Ph. Matile,F. Winkenbach +1 more
Journal ArticleDOI
The Metabolism of Oat Leaves during Senescence
TL;DR: It is deduced that an iron-containing system plays an essential part in the initiation of the senescence process of Avena seedlings.
Journal ArticleDOI
Antagonisms between Kinetin and Amino Acids: Experiments on the Mode of Action of Cytokinins.
TL;DR: In this article, the maintenance of chlorophyll in darkened first leaves of oats was used as a bioassay for cytokinins in pea (Pisum sativum) roots.
Journal ArticleDOI
Proteases of senescing oat leaves: I. Purification and general properties.
TL;DR: Two proteases active in the senescing first leaves of oat seedlings (Avena sativa cv. Victory) have been purified approximately 500-fold by a combination of ammonium sulfate precipitation, affinity chromatography on hemoglobin-Sepharose, and ion exchange Chromatography on DEAE-Sephadex.
Journal ArticleDOI
Isolation from Avena leaf tissues of a nuclease with the same type of specificity towards RNA and DNA accumulation of the enzyme during leaf senescence
TL;DR: The enzyme was shown to be an endonuclease which liberates nucleoside 5′-phosphates from both RNA and DNA and has a pronounced preference for the cleavage of bonds adjacent to adenine in RNA as well as DNA.
References
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Journal Article
Protein Measurement with the Folin Phenol Reagent
TL;DR: Procedures are described for measuring protein in solution or after precipitation with acids or other agents, and for the determination of as little as 0.2 gamma of protein.
Journal ArticleDOI
A study of the conditions and mechanism of the diphenylamine reaction for the colorimetric estimation of deoxyribonucleic acid
TL;DR: The present study arose from the observation that a more intense colour was sometimes produced if, instead of being heated at 1000 for 10 min., the reaction mixture was allowed to stand overnight at room temperature.
Journal ArticleDOI
Template properties of liver chromatin
Keiji Marushige,James Bonner +1 more
TL;DR: Kinetic analysis of the interaction of template and RNA polymerase indicates that the polymer enzyme binds equally well to DNA and to chromatin, but that in the latter, polymerase bound to a portion of the polymerase binding sites is prevented, by the presence of histone, from the execution of the transcription act.
Journal ArticleDOI
The effect of kinetin on nucleic acids and nucleases of excised barley leaves.
B I Srivastava,G Ware +1 more
TL;DR: The results of the investigation into the effect of kinetin on the amount of RNA, DNA and chlorophyll, on the rate of incorporation of p32 into RNA and DNA, and on the activity of soluble ribonuclease and deoxyrib onuclease in excised barley leaves are described.
Book ChapterDOI
Cytokinins in Plants
TL;DR: It is important to note that cytokinins are also transported through the xylem in intact plants, as cytokinin-like compounds have been detected in the sap of several plants.
Related Papers (5)
On the nature of increase in ribonuclease activity in mechanically damaged tobacco leaf tissues
G. Bagi,G.L. Farkas +1 more