Journal ArticleDOI
Increased Agrobacterium-mediated transformation and rooting efficiencies in canola (Brassica napus L.) from hypocotyl segment explants
V. Cardoza,Charles Neal Stewart +1 more
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TLDR
An efficient protocol for the production of transgenic Brassica napus cv.Abstract:
An efficient protocol for the production of transgenic Brassica napus cv. Westar plants was developed by optimizing two important parameters: preconditioning time and co-cultivation time. Agrobacterium tumefaciens-mediated transformation was performed using hypocotyls as explant tissue. Two variants of a green fluorescent protein (GFP)-encoding gene--mGFP5-ER and eGFP--both under the constitutive expression of the cauliflower mosaic virus 35S promoter, were used for the experiments. Optimizing the preconditioning time to 72 h and co-cultivation time with Agrobacterium to 48 h provided the increase in the transformation efficiency from a baseline of 4% to 25%. With mGFP5-ER, the transformation rate was 17% and with eGFP it was 25%. Transgenic shoots were selected on 200 mg/l kanamycin. Rooting efficiency was 100% on half-strength Murashige and Skoog medium with 10 g/l sucrose and 0.5 mg/l indole butyric acid in the presence of kanamycin.read more
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Agrobacterium-mediated transformation of chickpea (Cicer arietinum L.) with Bacillus thuringiensis cry1Ac gene for resistance against pod borer insect Helicoverpa armigera
TL;DR: Combination of these physical and physiological conditions during agroinoculation, co-cultivation and selection on kanamycin were found to be critical determinants resulting into increased competence for regeneration and co-transformation frequency of cry1Ac and npt II and elimination of escapes.
Journal ArticleDOI
Invited review: brassica biotechnology: progress in cellular and molecular biology
Vinitha Cardoza,C. Neal Stewart +1 more
TL;DR: The use of molecular markers in marker-assisted selection and breeding, transformation technology for the introduction of desirable traits, and a comparative analysis of these as well as their future prospects are important parts of the current research that is reviewed.
Agrobacterium-mediated transformation of plants: emerging factors that influence efficiency
TL;DR: Evidence that Agrobacterium density, co-culture medium, antibiotic and selectable marker influence T-DNA delivery and integration and stable transformation of plants were also presented.
Journal ArticleDOI
Pod shatter-resistant Brassica fruit produced by ectopic expression of the FRUITFULL gene
TL;DR: It is shown that ectopic expression of the Arabidopsis FRUITFULL gene in Brassica juncea is sufficient to produce pod shatter-resistant Brassica fruit and that the genetic pathway leading to valve margin specification is conserved betweenArabidopsis and Brassica.
Journal ArticleDOI
Brassica carinata – a new molecular farming platform for delivering bio‐industrial oil feedstocks: case studies of genetic modifications to improve very long‐chain fatty acid and oil content in seeds
David C. Taylor,Kevin C. Falk,C. Don Palmer,Joe K. Hammerlindl,Vivijan Babic,Elzbieta Mietkiewska,A. S. Jadhav,Elizabeth-France Marillia,Tammy Francis,Travis Hoffman,E. Michael Giblin,Vesna Katavic,Wilfred A. Keller +12 more
TL;DR: This collaborative B. carinata breeding/germplasm development/value‐added molecular modification effort will not only contribute to the development of renewable feedstocks for the emerging Canadian bioeconomy (biorefinery/bioproducts), but also promises to generate positive economic and environmental benefits.
References
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Journal ArticleDOI
A revised medium for rapid growth and bio assays with tobacco tissue cultures
Toshio Murashige,Folke Skoog +1 more
TL;DR: In vivo redox biosensing resolves the spatiotemporal dynamics of compartmental responses to local ROS generation and provide a basis for understanding how compartment-specific redox dynamics may operate in retrograde signaling and stress 67 acclimation in plants.
Modern methods of plant analysis
Journal ArticleDOI
Removal of a cryptic intron and subcellular localization of green fluorescent protein are required to mark transgenic Arabidopsis plants brightly
TL;DR: The green fluorescent protein (GFP) from the jellyfish Aequorea victoria is finding wide use as a genetic marker that can be directly visualized in the living cells of many heterologous organisms as discussed by the authors.
Journal ArticleDOI
Identification of the signal molecules produced by wounded plant cells that activate T-DNA transfer in Agrobacterium tumefaciens
TL;DR: It is shown here that Agrobacterium tumefaciens virulence (Vir) gene expression is activated specifically by the plant molecules acetosyringone (AS) and α-hydroxyacetosyringsone (OH-AS).
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A revised medium for rapid growth and bio assays with tobacco tissue cultures
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