Influenza virion RNA-dependent RNA polymerase: stimulation by guanosine and related compounds.
D McGeoch,N Kitron +1 more
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TLDR
Evidence is presented that the stimulation represents preferential initiation of genome complementary RNA chains with guanosine, and RNA made in the stimulated reaction behaves as complementary RNA in annealing kinetic studies, as does RNA labeled with [3-H]guanosine.Abstract:
The activity of RNA-dependent RNA polymerase of several influenza viruses is stimulated by guanosine. Depending upon the virus strain used, the stimulation of initial reaction rate is up to 10-fold. 5'-GMP, 3',5'-cyclic GMP, and 5'-GDP show lesser stimulation effects. No other nucleosides of 5'-NMPs stimulate, but the dinucleoside monophosphates GpG and GpC show large stimulations. We present evidence that the stimulation represents preferential initiation of genome complementary RNA chains with guanosine: (i) [3-H] guanosine is incorporated specifically at the 5'terminus of RNA in polymerase reaction mixes in vitro. (ii) This incorporation reaction has several properties similar to those of the virion polymerase elongation reaction. (iii) RNA made in the stimulated reaction behaves as complementary RNA in annealing kinetic studies, as does RNA labeled with [3-H]guanosine.read more
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The synthesis of Sendai virus polypeptides in infected cells
Robert A. Lamb,Robert A. Lamb,Brian W. J. Mahy,Brian W. J. Mahy,Purnell W. Choppin,Purnell W. Choppin +5 more
TL;DR: The results of pulse and pulse-chase experiments suggest that large, polyprotein precursors are not involved in Sendai virus replication, and that polypeptides are synthesized from monocistronic messenger RNA species.
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TL;DR: Ribavirin 5′-triphosphate (RTP), derived from the broad-spectrum antiviral compound ribavirin (Virazole), can selectively inhibit influenza virus ribonucleic acid polymerase in a cell-free assay.
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Influenza viral mRNA contains internal N6-methyladenosine and 5'-terminal 7-methylguanosine in cap structures.
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Inhibition of cap (m7GpppXm)-dependent endonuclease of influenza virus by 4-substituted 2,4-dioxobutanoic acid compounds.
Joanne E. Tomassini,Harold G. Selnick,M E Davies,M. E. Armstrong,J. Baldwin,M Bourgeois,J C Hastings,Daria J. Hazuda,John A. Lewis,Mcclements William L +9 more
TL;DR: A series of 4-substituted 2,4-dioxobutanoic acid compounds has been identified as selective inhibitors of endonucleolytic processing in both influenza A and B viruses, which provides a unique target for the development of antiviral agents for influenza viruses.
Book ChapterDOI
Expression and Replication of the Influenza Virus Genome
TL;DR: The genome of influenza A viruses is composed of eight segments of negative polarity and in infected cells, these virion RNAs are both transcribed into messenger RNAs (mRNAs) and replicated.
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