Journal ArticleDOI
Initiation of eukaryotic DNA replication in vitro
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TLDR
The system capable of replicating plasmid DNAs containing the SV40 origin of DNA replication in vitro is a paradigm for studies on the replicate of other virus DNAs and the replication of cellular chromosomes.Abstract:
Recent advances in our understanding of the mechanism and regulation of eukaryotic DNA replication have been expedited by the use of cell-free systems capable of initiation of DNA replication. The system capable of replicating plasmid DNAs containing the SV40 origin of DNA replication in vitro is a paradigm for studies on the replication of other virus DNAs and the replication of cellular chromosomes. This review outlines some of the contemporary issues and developments related to this complex problem.read more
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Journal ArticleDOI
The p21 inhibitor of cyclin-dependent kinases controls DNA replication by interaction with PCNA
TL;DR: It is shown that p21 directly inhibits PCNA-dependent DNA replication in the absence of a cyclin/CDK, and blocks the ability of PCNA to activateDNA polymerase δ, the principal replicative DNA polymerase.
Journal ArticleDOI
ATP-dependent recognition of eukaryotic origins of DNA replication by a multiprotein complex.
Stephen P. Bell,Bruce Stillman +1 more
TL;DR: It is proposed that the origin recognition complex acts as the initiator protein for S. cerevisiae origins of DNA replication, and specific DNA binding by theorigin recognition complex is dependent upon the addition of ATP.
Journal ArticleDOI
DNA Replication Fidelity
TL;DR: Current understanding of replication fidelity is reviewed, with emphasis on structural and biochemical studies of DNA polymerases that provide new insights into the importance of hydrogen bonding, base pair geometry, and substrate-induced conformational changes to fidelity.
Journal ArticleDOI
The dna replication fork in eukaryotic cells
Shou Waga,Bruce Stillman +1 more
TL;DR: Biochemical studies, principally of plasmid DNAs containing the Simian Virus 40 origin of DNA replication, and yeast genetic studies have uncovered the fundamental mechanisms of replication fork progression.
Journal ArticleDOI
Purification and characterization of CAF-I, a human cell factor required for chromatin assembly during DNA replication in vitro
Susan Smith,Bruce Stillman +1 more
TL;DR: The purification and characterization of a replication-dependent chromatin assembly factor (CAF-I) from the nuclei of human cells is described, and the reaction described herein reflects the process of chromatin formation during DNA replication in vivo.