Journal ArticleDOI
Interferences by sulfhydryl, disulfide reagents and potassium ions on protein determination by Lowry's method.
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TLDR
The concentrations ofulfhydryl, disulfide reagents, and also potassium ions interfere with the protein determination by Lowry's method, but at moderate concentrations this interference can be overcome by running appropriate blanks.About:
This article is published in Analytical Biochemistry.The article was published on 1970-07-01. It has received 72 citations till now.read more
Citations
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Journal ArticleDOI
A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding
TL;DR: This assay is very reproducible and rapid with the dye binding process virtually complete in approximately 2 min with good color stability for 1 hr with little or no interference from cations such as sodium or potassium nor from carbohydrates such as sucrose.
Journal ArticleDOI
Assay of proteins in the presence of interfering materials.
TL;DR: The Lowry protein assay is a sensitive but highly nonspecific procedure that has been modified so that protein can be assayed in the presence of interfering chemicals.
Journal ArticleDOI
A rapid, sensitive, and specific method for the determination of protein in dilute solution
TL;DR: This protein assay is described in which the sample is precipitated with trichloroacetic acid in the presence of sodium dodecylsulfate, filtered off on a Millipore membrane and stained with Amidoschwarz 10B, and its absorbance determined at 630 nm.
Journal ArticleDOI
Review of the Folin phenol protein quantitation method of Lowry, Rosebrough, Farr and Randall.
TL;DR: The method of Lowry and coworkers combined the use of copper, as suggested by Herriott, with the Folin phenol method, which originated from the work of Wu, to produce a more reliable and sensitive protein analysis.
Book ChapterDOI
Protein determination in membrane and lipoprotein samples: manual and automated procedures.
TL;DR: By adding sodium dodecyl sulfate to the alkali reagent, samples can be assayed directly without prior solubilization or delipidation and quantitation of protein in the presence of sucrose and EDTA is facilitated.
References
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Journal Article
Protein Measurement with the Folin Phenol Reagent
TL;DR: Procedures are described for measuring protein in solution or after precipitation with acids or other agents, and for the determination of as little as 0.2 gamma of protein.
Journal ArticleDOI
Influence of sucrose on protein determination by the Lowry procedure
B. P. Gerhardt,Harry Beevers +1 more
Journal ArticleDOI
Sucrose interference in the assay of enzymes and protein.
TL;DR: Sucrose has been found to interfere with the estimation of protein and with the assay of a variety of soluble and particulate enzymes.
Journal ArticleDOI
The subcellular distribution and properties of hexokinases in the guinea-pig cerebral cortex
TL;DR: Hexokinase activities were estimated in primary subcellular fractions from guinea-pig cerebral cortex and in sucrose-density-gradient subfractions of the mitochondrial and microsomal fractions and no evidence was obtained for the presence of a high-K(m) glucokinase in the brain.
Related Papers (5)
Protein determination by Lowry's method in the presence of sulfhydryl reagents.
Paul J. Geiger,Samuel P. Bessman +1 more
Influence of sucrose on protein determination by the Lowry procedure
B. P. Gerhardt,Harry Beevers +1 more