Isoelectric focusing of proteins in the native and denatured states. Anomalous behaviour of plasma albumin
M. R. Salaman,A. R. Williamson +1 more
TLDR
Isoelectric focusing of proteins in urea appears to be useful in the study of various aspects of protein structure and demonstrates heterogeneity in bovine plasma albumin based on primary-sequence differences.Abstract:
1. An analytical technique of isoelectric focusing in thin layers of polyacrylamide gel has been used to determine the isoelectric point, pI, of several proteins in the presence and in the absence of concentrated urea. 2. The presence of urea did not greatly affect pI except for bovine plasma albumin, where an increase of approx. 1pH unit was found. 3. Evidence is presented that this change in the pI of bovine plasma albumin is due to the normalization of certain ionizable groups on unfolding of the protein in urea. 4. Evidence is also presented that prolonged exposure of bovine plasma albumin to urea results in intramolecular disulphide interchange and that, on removal of urea, the new patterns of disulphide bonding stabilize abnormal conformations with pI values intermediate between those of the native and denatured states. 5. The studies demonstrate heterogeneity in bovine plasma albumin based on primary-sequence differences. 6. Isoelectric focusing of proteins in urea appears to be useful in the study of various aspects of protein structure.read more
Citations
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Journal ArticleDOI
The use of analytical isoelectric focusing for detection and identification of beta-lactamases.
TL;DR: Isoelectric focusing can be used to observe the transfer of beta-lactamases carried by R factors and it is shown that R that have not been distinguished from one another biochemically or immunologically can be differentiated by isoelectric focusing.
Journal ArticleDOI
Isoelectric points and molecular weights of proteins.
TL;DR: Proteins with known isoelectric points (pI), as determined by isoeLECTric focusing, have been tabulated and, in the case of proteins displaying microheterogeneity, the major components have been indicated.
Journal ArticleDOI
An α-d-Galactosyl-binding Lectin from Bandeirae simplicifolia Seeds ISOLATION BY AFFINITY CHROMATOGRAPHY AND CHARACTERIZATION
TL;DR: It is suggested that Bandeirae simplicifolia lectin, the first α-d-galactopyranosyl binding lectin (anti-B lectin) to be purified and characterized, will be valuable not only as a serological reagent, but in studies of polysaccharides, glycoproteins, and cell membrane structure.
Journal ArticleDOI
Neutrophil attractant/activation protein-1 (NAP-1 [interleukin-8]).
TL;DR: The wide spectrum of cell sources and production stimuli suggests that NAP-1 mediates neutrophil recruitment in host defense and disease.
Book ChapterDOI
Some aspects of the structure and conformational properties of serum albumin
TL;DR: Two general aspects of serum albumin structure and function are considered: the heterogeneity and microheterogeneity of the protein and the conformational transformations that the protein undergoes in solution.
References
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Journal ArticleDOI
Tissue sulfhydryl groups
TL;DR: A water-soluble (at pH 8) aromatic disulfide [5,5′-dithiobis(2-nitrobenzoic acid] has been synthesized and shown to be useful for determination of sulfhydryl groups.
Journal ArticleDOI
Isoelectric fractionation, analysis, and characterization of ampholytes in natural pH gradients. IV. Further studies on the resolving power in connection with separation of myoglobins.
Vesterberg O,Svensson H +1 more
Journal ArticleDOI
Isoelectric Focusing in Polyacrylamide Gel and its Application to Immunoglobulins
TL;DR: In this form the technique has been successfully applied to studies on the heterogeneity of myoglobins, cytochrome c6, lactoperoxidase7 and a number of other proteins (see review by Haglund8).
Journal ArticleDOI
Reactivity of sperm whale metmyoglobin towards hydrogen ions and p-nitrophenyl acetate.
TL;DR: The first of a projected series of studies addressed to these closely related questions is presented in this article, where the authors studied the equilibria of metmyoglobin with hydrogen ions, with greatest emphasis on the pH range below 9.
Journal ArticleDOI
Amino acid composition of human and bovine serum mercaptalbumins.
P.F. Spahr,John T. Edsall +1 more
TL;DR: Test the belief that the amino acid composition of the two fractions was nearly identical, and report the results, consider some of their implications, and compare the findings with those of other workers.
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