Isolation and characterization of a mammalian gene encoding a high-affinity cAMP phosphodiesterase
TLDR
It is shown that the DPD protein is a high-affinity cAMP-specific phosphodiesterase, a mutant form of the RAS2 gene analogous to an oncogenic mutant of the human HRAS gene.Abstract:
A rat brain cDNA library has been constructed in a Saccharomyces cerevisiae expression vector and used to isolate genes that can function in yeast to suppress the phenotypic effects of RAS2val19, a mutant form of the RAS2 gene analogous to an oncogenic mutant of the human HRAS gene. One cDNA, DPD, was cloned and its genetic and biochemical properties were characterized. A DPD product would share 80% amino acid sequence identity with the Drosophila melanogaster dunce-encoded protein over an extended region. We have shown that the DPD protein is a high-affinity cAMP-specific phosphodiesterase.read more
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The ubiquitinproteasome pathway is required for processing the NF-κB1 precursor protein and the activation of NF-κB
TL;DR: The ubiquitin-proteasome pathway has been shown to play an essential role in two proteolytic processes required for activation of the transcription factor NF-κB as discussed by the authors.
Journal ArticleDOI
Human cyclin E, a nuclear protein essential for the G1-to-S phase transition.
TL;DR: It is found that mammalian cells express two forms of cyclin E protein which differ from each other by the presence or absence of a 15-amino-acid amino-terminal domain, and cyclins E and D1 control two different transitions within the human cell cycle.
Journal ArticleDOI
Biochemistry and Physiology of Cyclic Nucleotide Phosphodiesterases: Essential Components in Cyclic Nucleotide Signaling
Marco Conti,Joseph A. Beavo +1 more
TL;DR: Some of the major concepts related to the understanding of PDE function and regulation are reviewed including the structure of catalytic and regulatory domains and arrangement in holoenzymes, the nature and function of negative and positive feedback circuits that have been conserved in PDEs from prokaryotes to human, and the emerging association of mutant PDE alleles with inherited diseases.
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Isolation of three novel human cyclins by rescue of G1 cyclin (cln) function in yeast
TL;DR: Three novel genes have been identified, which are sufficiently distantly related to the other members of the cyclin family and to each other as to constitute three new classes of cyclins.
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The NF1 locus encodes a protein functionally related to mammalian GAP and yeast IRA proteins.
R. Ballester,Douglas A. Marchuk,Douglas A. Marchuk,Mark S. Boguski,Ann M. Saulino,Ann M. Saulino,Roxanne Letcher,Roxanne Letcher,Michael Wigler,Francis S. Collins,Francis S. Collins +10 more
TL;DR: It is indicated that the NF1 gene product can interact with RAS proteins and structural and functional similarities and differences among the GAP, IRA1, IRA2, and NF1 proteins are demonstrated.
References
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Book
DNA cloning : a practical approach
TL;DR: The construction and characterization of vaccinia virus recombinants expressing foreign genes Bovine papilloma virus DNA: A eukaryotic cloning vector is studied.