Kinetics of hydrolysis of fructooligosaccharides in mineral-buffered aqueous solutions: influence of pH and temperature.

TLDR: The three oligomers were found to be degraded mainly under acidic conditions, and at the highest temperature value (120 degrees C), a quick and complete acid degradation of each FOS was observed.

Abstract: High-performance anion exchange chromatography coupled with a pulsed amperometric detection system (HPAEC−PAD) was used to evaluate the extent of chemical hydrolysis of three fructooligosaccharides (FOS) including 1-kestose (β-D-Fru-(2→1)2-α-D-glucopyranoside, GF2), nystose (β-D-Fru-(2→1)3-α-D-glucopyranoside, GF3), and fructofuranosylnystose (β-D-Fru-(2→1)4-α-D-glucopyranoside, GF4). A kinetic study was carried out at 80, 90, 100, 110, and 120 °C in aqueous solutions buffered at pH values of 4.0, 7.0, and 9.0. Under each experimental condition, the determination of the respective amounts of reactants and hydrolysis products showed that FOS hydrolysis obeyed pseudo-first-order kinetics as the extent of hydrolysis, which decreased at increasing pH values, increased with temperature. The three oligomers were found to be degraded mainly under acidic conditions, and at the highest temperature value (120 °C), a quick and complete acid degradation of each FOS was observed. Using the Arrhenius equation, rate con...