Book ChapterDOI
Label-free Detection of Proteins with Surface-functionalized Silicon Nanowires
R. E. Chee,J. H. Chua,Ajay Agarwal,S. M. Wong,G. J. Zhang +4 more
- pp 838-841
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TLDR
A proof-of-principle protein detection using biotinylated SiNWs for the detection of ultralow concentrations of streptavidin with a maximum sensitivity of up to 10 fM is demonstrated.Abstract:
Semiconducting silicon nanowires (SiNWs) have shown great potential for the electrical detection of human disease biomarkers. The technique for label-free detection using SiNWs allows for fast, efficient and inexpensive detection of biomarkers, unlike current time-consuming methodologies such as fluorescence ELISA or western blot. We demonstrate a proof-of-principle protein detection using biotinylated SiNWs for the detection of ultralow concentrations of streptavidin with a maximum sensitivity of up to 10 fM. Real-time detection of human cardiac troponin T using antibody-functionalized SiNWs is also presented. The possibility of large scale integration paves the way towards the simultaneous detection of a large number of protein biomarkers within a single chip and is of importance to the development of diagnostic tools as well as point-of-care applications.read more
Citations
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Journal ArticleDOI
Electrochemical biosensors based on nanofibres for cardiac biomarker detection: A comprehensive review.
TL;DR: The primary aim of this review is to emphasise the prospects for nanofibres for the future development of biosensors in diagnosis of CVDs as well as considering how to improve their characteristics for application in medicine.
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A Review of Characterization and Quantification Tools for Microbiologically Influenced Corrosion in the Oil and Gas Industry: Current and Future Trends
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Semiconductor Electronic Label-Free Assay for Predictive Toxicology
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Facile fabrication of a silicon nanowire sensor by two size reduction steps for detection of alpha-fetoprotein biomarker of liver cancer
TL;DR: In this article, a facile technique that only uses conventional micro-techniques and two size reduction steps to fabricate wafer-scale silicon nanowire (SiNW) with widths of 200 nm was presented.
Proceedings ArticleDOI
Ultrasensitive biomolecular assays with amplifying nanowire FET biosensors
TL;DR: The ultrasensitive electronic biomolecular assays enabled by the novel amplifying nanowire field-effect transistor (nwFET) biosensors are reviewed, demonstrating an ultralow limit of detection and concurrently high output current level for the first time.
References
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Journal ArticleDOI
Nanowire Nanosensors for Highly Sensitive and Selective Detection of Biological and Chemical Species
TL;DR: The small size and capability of these semiconductor nanowires for sensitive, label-free, real-time detection of a wide range of chemical and biological species could be exploited in array-based screening and in vivo diagnostics.
Journal ArticleDOI
Multiplexed electrical detection of cancer markers with nanowire sensor arrays.
TL;DR: Highly sensitive, label-free, multiplexed electrical detection of cancer markers using silicon-nanowire field-effect devices in which distinct nanowires and surface receptors are incorporated into arrays opens up substantial possibilities for diagnosis and treatment of cancer and other complex diseases.
Journal ArticleDOI
Label-free immunodetection with CMOS-compatible semiconducting nanowires
Eric Stern,James F. Klemic,David A. Routenberg,Pauline N. Wyrembak,Daniel B. Turner-Evans,Andrew D. Hamilton,David A. LaVan,Tarek M. Fahmy,Mark A. Reed +8 more
TL;DR: This work reports an approach that uses complementary metal oxide semiconductor (CMOS) field effect transistor compatible technology and hence demonstrates the specific label-free detection of below 100 femtomolar concentrations of antibodies as well as real-time monitoring of the cellular immune response.
Journal ArticleDOI
Direct ultrasensitive electrical detection of DNA and DNA sequence variations using nanowire nanosensors
TL;DR: Two-terminal silicon nanowire electronic devices that function as ultrasensitive and selective detectors of DNA are reported that show that detection can be carried out to at least the tens of femtomolar range.
Journal ArticleDOI
Electrical detection of single viruses
Fernando Patolsky,Gengfeng Zheng,Oliver Hayden,Melike Lakadamyali,Xiaowei Zhuang,Charles M. Lieber +5 more
TL;DR: Direct, real-time electrical detection of single virus particles with high selectivity by using nanowire field effect transistors is reported, suggesting potential for simultaneous detection of a large number of distinct viral threats at the single virus level.