Journal ArticleDOI
Label-free fluorescent detection of microRNA-155 based on synthesis of hairpin DNA-templated copper nanoclusters by etching (top-down approach)
Yasaman-Sadat Borghei,Morteza Hosseini,Morteza Hosseini,Mohammad Reza Ganjali,Mohammad Reza Ganjali,Saman Hosseinkhani +5 more
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TLDR
The nanobiosensor showed the potential of becoming a potential alternative tool for the detection of miRNA-155 in biomedical research and early clinical diagnostic studies.Abstract:
In this study, we have reported a new approach for sequence specific microRNA-155 (miRNA-155) detection by water soluble luminescent copper nanoclusters. The method is based on the shifts in the fluorescence emissions of oligonucleotide-templated copper nanoclusters (DNA-CuNCs) and can be regarded as the first instance of using fluorescent CuNCs for the detection of microRNA by forming DNA-RNA heteroduplex. The as-prepared CuNCs exhibited a strong fluorescence enhancement at 510 nm and a red shift (60 nm) when bonded to the target miRNA-155 (DNA-RNA heteroduplex formation). Under the optimal conditions, the fluorescent intensity of DNA-CuNCs increased by the increasing the target miRNA-155 with in a dynamic range from 5.0 × 10 −12 to 1.0 × 10 −8 M down to a detection limit (LOD) of 2.2 × 10 −12 M. The target miRNA is selectively discriminated from mismatched miRNA by this method. The method was further applied to the determination of miRNA spiked human serum samples. Overall, the nanobiosensor showed the potential of becoming a potential alternative tool for the detection of miRNA-155 in biomedical research and early clinical diagnostic studies.read more
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Journal ArticleDOI
Research advances in the detection of miRNA.
TL;DR: This review will provide guidelines for the development of follow-up miRNA detection methods with high sensitivity and specificity, and applicability to disease diagnosis and therapy.
Journal ArticleDOI
Ultrasensitive Fluorescent miRNA Biosensor Based on a "Sandwich" Oligonucleotide Hybridization and Fluorescence Resonance Energy Transfer Process Using an Ln(III)-MOF and Ag Nanoparticles for Early Cancer Diagnosis: Application of Central Composite Design
Ahmad Afzalinia,Mahdi Mirzaee +1 more
TL;DR: A novel, rapid, highly sensitive, and selective fluorescent biosensor, designed based on "sandwich-type" hybridization of oligonucleotides and the fluorescence resonance energy transfer (FRET) strategy, that senses and determines the MicroRNA-155 (mi RNA-155) expression levels as a cancer biomarker.
Journal ArticleDOI
Fluorescent turn on sensing of Caffeine in food sample based on sulfur-doped carbon quantum dots and optimization of process parameters through response surface methodology
Fatemeh Nemati,Morteza Hosseini,Morteza Hosseini,Rouholah Zare-Dorabei,Foad Salehnia,Mohammad Reza Ganjali,Mohammad Reza Ganjali +6 more
TL;DR: In this paper, an off-on fluorescent probe was designed for detection of caffeine in real media using sulfur-doped carbon quantum dots (S-CQDs) as probe.
Journal ArticleDOI
Paper based colorimetric detection of miRNA-21 using Ag/Pt nanoclusters.
Neda Fakhri,Shima Abarghoei,Mehdi Dadmehr,Morteza Hosseini,Hossein Sabahi,Mohammad Reza Ganjali +5 more
TL;DR: A novel paper based biosensor was fabricated to detect sub-micro molar concentrations of miRNA-21 based on peroxidase mimetic activity of DNA-templated Ag/Pt nanoclusters (DNA-Ag/PT NCs), which could catalyze the reaction of hydrogen peroxide and 3,3',5,5' tetramethylbenzidine (TMB), to produce a blue color.
Journal ArticleDOI
Copper nanoclusters: designed synthesis, structural diversity, and multiplatform applications.
Ani Baghdasaryan,Thomas Bürgi +1 more
TL;DR: The field of monolayer-protected metal nanoclusters, especially copper, with well-defined molecular structures and compositions, is relatively new, about two to three decades old as discussed by the authors.
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