Journal ArticleDOI
Large-scale production in Pichia pastoris of the recombinant vaccine Gavac against cattle tick.
Mario Canales,Antonio Enríquez,Eduardo Ramos,Deborah Cabrera,Hubert Dandie,Alejandro Soto,Viviana Falcón,Manuel Rodríguez,José de la Fuente +8 more
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TLDR
The development and large-scale production of the Gavac vaccine, the efficacy of the resulting product and the strategy followed in designing its production plant are reviewed.About:
This article is published in Vaccine.The article was published on 1997-03-01. It has received 171 citations till now. The article focuses on the topics: Pichia pastoris.read more
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Journal ArticleDOI
Heterologous protein expression in the methylotrophic yeast Pichia pastoris
TL;DR: This paper reviews the P. pastoris expression system: how it was developed, how it works, and what proteins have been produced and describes new promoters and auxotrophic marker/host strain combinations which extend the usefulness of the system.
Journal ArticleDOI
Recombinant protein expression in Pichia pastoris.
TL;DR: Major advances in the development of new strains and vectors, improved techniques, and the commercial availability of these tools coupled with a better understanding of the biology of Pichia species have led to this microbe’s value and power in commercial and research labs alike.
Journal ArticleDOI
A ten-year review of commercial vaccine performance for control of tick infestations on cattle.
José de la Fuente,Consuelo Almazán,Mario Canales,José M. Pérez de la Lastra,Katherine M. Kocan,Peter Willadsen +5 more
TL;DR: Commercial tick vaccines for cattle based on the Boophilus microplus Bm86 gut antigen have proven to be a feasible tick control method that offers a cost-effective, environmentally friendly alternative to the use of acaricides.
Journal ArticleDOI
Anti-tick vaccines.
TL;DR: There is now abundant evidence that vaccination with defined protein antigens is able to induce significant immunity to tick infestation, but under most circumstances the use of a tick vaccine as the single, stand alone control technology is likely to require more efficacious vaccines than those currently available.
Journal ArticleDOI
Production of recombinant proteins by methylotrophic yeasts
TL;DR: The methylotrophic yeasts Hansenula polymorpha, Pichia pastoris and Candida boidinii have been developed as production systems for recombinant proteins and are rapidly becoming the systems of choice for heterologous gene expression in yeast.
References
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Journal Article
Protein Measurement with the Folin Phenol Reagent
TL;DR: Procedures are described for measuring protein in solution or after precipitation with acids or other agents, and for the determination of as little as 0.2 gamma of protein.
Journal ArticleDOI
Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4
TL;DR: Using an improved method of gel electrophoresis, many hitherto unknown proteins have been found in bacteriophage T4 and some of these have been identified with specific gene products.
Journal Article
Cleavage of structural proteins during the assemble of the head of bacterio-phage T4
TL;DR: Using an improved method of gel electrophoresis, many hitherto unknown proteins have been found in bacteriophage T4 and some of these have been identified with specific gene products as mentioned in this paper.
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A low-viscosity epoxy resin embedding medium for electron microscopy.
TL;DR: A low-viscosity embedding medium based on ERL-4206 is recommended for use in electron microscopy and has a long pot life of several days and infiltrates readily because of its low viscosity.
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Commercialisation of a recombinant vaccine against Boophilus microplus.
TL;DR: The vaccine has been tested in the field, has been taken through the full registration process and is now in commercial use in Australia, and a related development has occurred in Cuba.