Luminescence switch-on detection of protein tyrosine kinase-7 using a G-quadruplex-selective probe
Sheng Lin,Wei Gao,Zeru Tian,Chao Yang,Lihua Lu,Jean-Louis Mergny,Jean-Louis Mergny,Chung-Hang Leung,Dik-Lung Ma +8 more
Reads0
Chats0
TLDR
A novel luminescent G-quadruplex-selective iridium(iii) complex was employed in a G- quadruplex -based detection assay for PTK7.Abstract:
A series of luminescent iridium(iii) complexes were synthesised and evaluated for their ability to act as luminescent G-quadruplex-selective probes. The iridium(iii) complex 9 [Ir(pbi)2(5,5-dmbpy)]PF6 (where pbi = 2-phenyl-1H-benzo[d]imidazole; 5,5-dmbpy = 5,5'-dimethyl-2,2'-bipyridine) exhibited high luminescence for G-quadruplex DNA compared to dsDNA and ssDNA, and was employed to construct a G-quadruplex-based assay for protein tyrosine kinase-7 (PTK7) in aqueous solution. PTK7 is an important biomarker for a range of leukemias and solid tumors. In the presence of PTK7, the specific binding of the sgc8 aptamer sequence triggers a structural transition and releases the G-quadruplex-forming sequence. The formation of the nascent G-quadruplex structure is then detected by the G-quadruplex-selective iridium(iii) complex with an enhanced luminescent response. Moreover, the application of the assay for detecting PTK7 in cellular debris and membrane protein extract was demonstrated. To our knowledge, this is the first G-quadruplex-based assay for PTK7.read more
Citations
More filters
Journal ArticleDOI
I and i
TL;DR: There is, I think, something ethereal about i —the square root of minus one, which seems an odd beast at that time—an intruder hovering on the edge of reality.
Journal ArticleDOI
Duplexed aptamers: history, design, theory, and application to biosensing
TL;DR: A framework for DAs based on ACEs is introduced, and this framework is used to distinguish DAs from other aptasensor formats and to categorize cis- and trans-DA designs, and to present future directions of DAs as ligand-responsive nucleic acids.
Journal ArticleDOI
Two-dimensional oriented growth of Zn-MOF-on-Zr-MOF architecture: A highly sensitive and selective platform for detecting cancer markers.
Nan Zhou,Fangfang Su,Chuanpan Guo,Linghao He,Zhankui Jia,Minghua Wang,Qiaojuan Jia,Zhihong Zhang,Siyu Lu +8 more
TL;DR: A novel strategy for obtaining ZnZr bimetallic MOFs via the MOF-on-MOF method and exploiting them as an aptasensor platform for detecting the cancer marker protein tyrosine kinase-7 (PTK7) is reported.
Journal ArticleDOI
Novel hybrid probe based on double recognition of aptamer-molecularly imprinted polymer grafted on upconversion nanoparticles for enrofloxacin sensing.
TL;DR: A novel luminescent "double recognition" imprinting cavities are formed after removal of ENR, displaying recognition properties superior to that of aptamer or traditional molecularly imprinting alone, and show great potential for wide-ranging application in bioassays.
Journal ArticleDOI
A long lifetime luminescent iridium(III) complex chemosensor for the selective switch-on detection of Al3+ ions
Wanhe Wang,Zhifeng Mao,Modi Wang,Li-Juan Liu,Daniel W. J. Kwong,Chung-Hang Leung,Dik-Lung Ma +6 more
TL;DR: A novel luminescent cyclometalated iridium(III) complex was synthesized and employed as a chemosensor for the detection of Al(3+) ions and displays a larger Stokes shift and a long lifetime luminescence that allow to detect Al( 3+) ions in fluorescent dye spiked strong fluorescence media and potentially be used in strongly autofluorescent biological samples.
References
More filters
Journal ArticleDOI
I and i
TL;DR: There is, I think, something ethereal about i —the square root of minus one, which seems an odd beast at that time—an intruder hovering on the edge of reality.
Journal ArticleDOI
Luminescent chemodosimeters for bioimaging.
TL;DR: Key Laboratory for Organic Electronics and Information Displays (KLOEID) and Institute of Advanced Materials (IAM), Nanjing University of Posts and Telecommunications, Nanjing 210046, P. R. China.
Journal ArticleDOI
Aptamers as therapeutics.
TL;DR: A series of aptamers currently in development may change how nucleic acid therapeutics are perceived and will increasingly find use in concert with other therapeutic molecules and modalities.