Journal ArticleDOI
Major haemolymph proteins inCeratitis capitata: Biosynthesis and secretion during development.
George Chrysanthis,Vassilis J. Marmaras,Constantin Christodoulou +2 more
- Vol. 190, Iss: 1, pp 33-39
TLDR
It is evident from the in vivo and organ culture studies, that the major haemolymph proteins are synthesized during the late larval stage and their synthesis declines abruptly during the stage of white pupae.Abstract:
The accumulation of major haemolymph proteins (a group of proteins immunologically related to Calliphorin) their biosynthesis in vivo and in organ culture as well as their secretion, has been studied during the late larval stages and white pupae of the Mediterranean fruit flyCeratitis capitata. The accumulation of major haemolymph proteins in the haemolymph, shows a twenty fold increase from the 4-day old larvae to the white pupae stage, while in the fat body there is only a seven fold increase. It is evident from the in vivo and organ culture studies, that the major haemolymph proteins are synthesized during the late larval stage and their synthesis declines abruptly during the stage of white pupae. It seems also that each polypeptide has its own characteristic developmental kinetics of synthesis. The major haemolymph proteins are synthesized in the fat body and are very quickly secreted into the haemolymph.read more
Citations
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Journal ArticleDOI
Translation of the mRNAs coding for the major hemolymph proteins of Ceratitis capitata in cell-free system: comparison of the translatable mRNA levels to the respective biosynthetic levels of the proteins in the fat body during development.
TL;DR: Four major hemolymph polypeptides (ceratitins) with molecular weights between 8.1 X 10(4) and 8.7 X 10 (4) daltons have been identified in the fat body of late Ceratitis capitata larvae and comparison of the biosynthetic patterns obtained suggests a linear relationship between messenger concentration and protein synthesis.
Journal ArticleDOI
Quaternary and subunit structure of Calliphora arylphorin as deduced from electron microscopy, electrophoresis, and sequence similarities with arthropod hemocyanin.
Jürgen Markl,Thorsten Burmester,Heinz Decker,Anette Savel-Niemann,J. Robin Harris,Michaela Süling,Ulrike Naumann,Klaus Scheller +7 more
TL;DR: A detailed model of the quaternary structure of Calliphora arylphorin is presented and it is uncertain whether protein II belongs to the hemocyanin superfamily or not.
Journal ArticleDOI
Adult expression of the Drosophila Lsp-2 gene
Helen Beneš,Ronda G. Edmondson,Pat Fink,Jana Kejzlarova-Lepesant,Jean-Antoine Lepesant,Joe P. Miles,David W. Spivey +6 more
TL;DR: It is reported that the LSP-2 polypeptide accumulates in the hemolymph throughout adult life as well, suggesting a differential regulation for expression of the Drosophila Lsp-2 gene in adults and larvae.
Journal ArticleDOI
Isolation of two cDNA clones coding for larval hemolymph proteins of Galleria mellonella
TL;DR: Galleria mellonella a group of four larval hemolymph proteins (LHP) (74, 76, 81 and 82 kDa), which had been earlier shown to be storage proteins, exhibit a stage-specific synthetic pattern and are tentatively identified as two LHP-specific cDNA clones.
Journal ArticleDOI
Morphogenesis and cuticular markers during the larval-pupal transformation of the medfly Ceratitis capitata
TL;DR: Changes in morphology during early metamorphosis of the medfly, Ceratitis capitata (Wied.) (Tephritidae) were correlated with biochemical differentiation events and the definitive profile of pupal cuticle proteins was attained together with the establishment of adult body proportions.
References
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Journal Article
Protein Measurement with the Folin Phenol Reagent
TL;DR: Procedures are described for measuring protein in solution or after precipitation with acids or other agents, and for the determination of as little as 0.2 gamma of protein.
Journal ArticleDOI
Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4
TL;DR: Using an improved method of gel electrophoresis, many hitherto unknown proteins have been found in bacteriophage T4 and some of these have been identified with specific gene products.
Journal ArticleDOI
Quantitative film detection of 3H and 14C in polyacrylamide gels by fluorography.
TL;DR: It is demonstrated that pre-exposure of the film to a brief flash of light greatly increases the sensitivity of fluorography, because it corrects the non-linear relationship between radioactivity of the sample and absorbance of theFilm image.
Journal ArticleDOI
Measurement of the incorporation of radioactive amino acids into protein by a filter-paper disk method
Rusty J. Mans,G.David Novelli +1 more
TL;DR: A rapid and sensitive method has been described for the measurement of amino acid incorporation in cell-free systems and has been applied to both C14- and H3-labeled proteins.
Journal ArticleDOI
The soluble proteins of developing: Calliphora erythrocephala, particularly calliphorin, and similar proteins in other insects
E.A. Munn,G.D. Greville +1 more
TL;DR: Eggs, larvae, pupae, pharate adults, and emerged adults of the blowfly Calliphora erythrocephala have been examined for the presence of calliphorin, a protein of molecular weight 540,000; immunodiffusion, immunoelectrophoretic, ultracentrifugal, and electron-microscopic procedures were used.
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