Journal ArticleDOI
Mammalian Ras interacts directly with the serine/threonine kinase Raf
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TLDR
Raf interacts with wild-type and activated Ras, but not with an effector domain mutant of Ras or with a dominant-interfering Ras mutant, and this interaction is dependent on GTP bound to Ras.About:
This article is published in Cell.The article was published on 1993-07-16. It has received 1979 citations till now. The article focuses on the topics: Rap GTP-binding protein & SH3 domain.read more
Citations
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Mitogen-activated protein (MAP) kinase pathways: regulation and physiological functions.
Gray W. Pearson,Fred L. Robinson,Tara Beers Gibson,Bing E. Xu,Mahesh Karandikar,Kevin S. Berman,Melanie H. Cobb +6 more
TL;DR: Nonenzymatic mechanisms that impact MAP kinase functions and findings from gene disruption studies are highlighted and particular emphasis is on ERK1/2.
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Regulation of myosin phosphatase by Rho and Rho-associated kinase (Rho-kinase)
Kazushi Kimura,Masaaki Ito,Mutsuki Amano,Kazuyasu Chihara,Yuko Fukata,Masato Nakafuku,Bunpei Yamamori,Jianhua Feng,Takeshi Nakano,Katsuya Okawa,Akihiro Iwamatsu,Kozo Kaibuchi +11 more
TL;DR: Rho appears to inhibit myosin phosphatase through the action of Rho-kinase, which is activated by GTP·RhoA, phosphorylation of MBS and MLC in NIH 3T3 cells.
Journal ArticleDOI
Genomic Libraries and a Host Strain Designed for Highly Efficient Two-Hybrid Selection in Yeast
TL;DR: A novel multienzyme approach was used to generate a set of highly representative genomic libraries from S. cerevisiae and a unique host strain was created that contains three easily assayed reporter genes, each under the control of a different inducible promoter.
Journal ArticleDOI
A synthetic inhibitor of the mitogen-activated protein kinase cascade.
TL;DR: Results indicate that the MAPK pathway is essential for growth and maintenance of the ras-transformed phenotype and PD 098059 is an invaluable tool that will help elucidate the role of theMAPK cascade in a variety of biological settings.
Journal ArticleDOI
Small GTP-Binding Proteins
TL;DR: In this review, functions of small G proteins and their modes of activation and action are described.
References
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Journal ArticleDOI
A novel genetic system to detect protein-protein interactions.
Stanley Fields,Ok-kyu Song +1 more
TL;DR: A novel genetic system to study protein-protein interactions between two proteins by taking advantage of the properties of the GAL4 protein of the yeast Saccharomyces cerevisiae, which may be applicable as a general method to identify proteins that interact with a known protein by the use of a simple galactose selection.
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The GTPase superfamily: conserved structure and molecular mechanism
TL;DR: GTPases are conserved molecular switches, built according to a common structural design, and rapidly accruing knowledge of individual GTPases—crystal structures, biochemical properties, or results of molecular genetic experiments—support and generate hypotheses relating structure to function in other members of the diverse family of GTPase.
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The GTPase superfamily: a conserved switch for diverse cell functions
TL;DR: A molecular switch is a molecular switch whose "on" and "off" states are triggered by binding and hydrolysis of GTP as discussed by the authors. But the mechanism in myriad versions of the switch can be traced back to a single primordial protein.
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High efficiency transformation of intact yeast cells using single stranded nucleic acids as a carrier.
Robert H. Schiestl,R D Gietz +1 more
TL;DR: A method, using LiAc to yield competent cells, is described that increased the efficiency of genetic transformation of intact cells of Saccharomyces cerevisiae to more than 1 × 105 transformant per microgram of vector DNA and to 1.5% transformants per viable cell.
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Raf-1 activates MAP kinase-kinase.
John M. Kyriakis,Harald App,Xian-feng Zhang,Papia Banerjee,David L. Brautigan,Ulf R. Rapp,Joseph Avruch +6 more
TL;DR: Results indicate that c-Raf-1 is an immediate upstream activator of MAPK-K in vivo, the first physiological substrate of the c-raf-l protooncogene product to be identified.