Journal ArticleDOI
Massively parallel manipulation of single cells and microparticles using optical images.
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TLDR
An optical image-driven dielectrophoresis technique that permits high-resolution patterning of electric fields on a photoconductive surface for manipulating single particles and requires 100,000 times less optical intensity than optical tweezers is presented.Abstract:
The ability to manipulate biological cells and micrometre-scale particles plays an important role in many biological and colloidal science applications. However, conventional manipulation techniques--including optical tweezers, electrokinetic forces (electrophoresis, dielectrophoresis, travelling-wave dielectrophoresis), magnetic tweezers, acoustic traps and hydrodynamic flows--cannot achieve high resolution and high throughput at the same time. Optical tweezers offer high resolution for trapping single particles, but have a limited manipulation area owing to tight focusing requirements; on the other hand, electrokinetic forces and other mechanisms provide high throughput, but lack the flexibility or the spatial resolution necessary for controlling individual cells. Here we present an optical image-driven dielectrophoresis technique that permits high-resolution patterning of electric fields on a photoconductive surface for manipulating single particles. It requires 100,000 times less optical intensity than optical tweezers. Using an incoherent light source (a light-emitting diode or a halogen lamp) and a digital micromirror spatial light modulator, we have demonstrated parallel manipulation of 15,000 particle traps on a 1.3 x 1.0 mm2 area. With direct optical imaging control, multiple manipulation functions are combined to achieve complex, multi-step manipulation protocols.read more
Citations
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Patent
Projection lens, projection device and optically-induced microparticle device
TL;DR: In this paper, a projection lens, a projection device and an optically-induced microparticle device are provided, which includes an aperture, a first and a second lens groups, disposed on a projection path of an image.
Dissertation
Optimal Control of Objects on the Micro- and Nano-Scale by Electrokinetic and Electromagnetic Manipulation: for Bio-Sample Preparation, Quantum Information Devices and Magnetic Drug Delivery
TL;DR: In this article, the authors present an approach to steer biological cells and nano-objects to nano meter precision by creating an electrokinetic fluid flow that carries all the particles from where they are to where they should be at each time step.
Proceedings ArticleDOI
OET meets acoustic tweezing
TL;DR: In this paper, a micromanipulation system based on dielectrophoretic and acoustophoretic particle tweezing is presented, where the combination of non-uniform electrical fields and standing pressure waves is applied to a microfluidic chip and provides essential functions including concentration, focussing, guiding, trapping and sorting of microparticles.
Journal ArticleDOI
Patterning of Particles and Live Cells at Single Cell Resolution.
TL;DR: A micromanipulator system is configured, in which a pneumatic microinjector is coupled to a holding pipette capable of physically isolating single particles and cells from different types, and positioning them with high accuracy in a predefined position, with a resolution smaller than 10 µm.
Journal ArticleDOI
Implementation of hybrid PDMS-graphite/Ag conductive material for flexible electronic devices and microfluidic applications
Yanfang Guan,Fengqian Xu,Xiaoliang Wang,Yanbo Hui,Jie Sha,Yong Tian,Zhongying Wang,Shixiong Zhang,Dali Chen,Liyan Yang +9 more
TL;DR: In this article, a conductive material consisting of polydimethylsiloxane (PDMS) and conductive inks (graphite or Ag) is used, which not only keeps the conductivity of the ink, but also improves the hydrophobicity of the PDMS and adhesiveness of the conductive ink.
References
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Journal ArticleDOI
A revolution in optical manipulation
TL;DR: This research presents the next generation of single-beam optical traps, which promise to take optical tweezers out of the laboratory and into the mainstream of manufacturing and diagnostics and even become consumer products.
Journal ArticleDOI
Optical trapping and manipulation of single cells using infrared laser beams
TL;DR: The use of infrared (IR) light is used to make much improved laser traps with significantly less optical damage to a variety of living cells, and new manipulative techniques using IR light are capable of producing large forces under damage-free conditions and improve the prospects for wider use of optical manipulation techniques in microbiology.
Journal ArticleDOI
Separation of Metallic from Semiconducting Single-Walled Carbon Nanotubes
TL;DR: This work has developed a method to separate metallic from semiconducting single-walled carbon nanotubes from suspension using alternating current dielectrophoresis, taking advantage of the difference of the relative dielectric constants of the two species with respect to the solvent.
Journal ArticleDOI
Dynamic holographic optical tweezers
TL;DR: In this article, the authors describe methods for creating large numbers of high-quality optical traps in arbitrary three-dimensional configurations and for dynamically reconfiguring them under computer control, allowing for mixed arrays of traps based on different modes of light, including optical vortices, axial line traps, optical bottles and optical rotators.
Journal ArticleDOI
Microfluidic sorting in an optical lattice
TL;DR: An optical sorter for microscopic particles that exploits the interaction of particles—biological or otherwise—with an extended, interlinked, dynamically reconfigurable, three-dimensional optical lattice, and can be applied in colloidal, molecular and biological research.