Journal ArticleDOI
Massively parallel manipulation of single cells and microparticles using optical images.
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TLDR
An optical image-driven dielectrophoresis technique that permits high-resolution patterning of electric fields on a photoconductive surface for manipulating single particles and requires 100,000 times less optical intensity than optical tweezers is presented.Abstract:
The ability to manipulate biological cells and micrometre-scale particles plays an important role in many biological and colloidal science applications. However, conventional manipulation techniques--including optical tweezers, electrokinetic forces (electrophoresis, dielectrophoresis, travelling-wave dielectrophoresis), magnetic tweezers, acoustic traps and hydrodynamic flows--cannot achieve high resolution and high throughput at the same time. Optical tweezers offer high resolution for trapping single particles, but have a limited manipulation area owing to tight focusing requirements; on the other hand, electrokinetic forces and other mechanisms provide high throughput, but lack the flexibility or the spatial resolution necessary for controlling individual cells. Here we present an optical image-driven dielectrophoresis technique that permits high-resolution patterning of electric fields on a photoconductive surface for manipulating single particles. It requires 100,000 times less optical intensity than optical tweezers. Using an incoherent light source (a light-emitting diode or a halogen lamp) and a digital micromirror spatial light modulator, we have demonstrated parallel manipulation of 15,000 particle traps on a 1.3 x 1.0 mm2 area. With direct optical imaging control, multiple manipulation functions are combined to achieve complex, multi-step manipulation protocols.read more
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Templated Self-Assembly of Particles
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TL;DR: Temple assembly can position particles even in the low-nanometer size regime, and it can do so efficiently for many particles in parallel as mentioned in this paper, which is the case for many nanoparticles in parallel.
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Scaling law analysis of electrohydrodynamics and dielectrophoresis for isomotive dielectrophoresis microfluidic devices
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Journal ArticleDOI
The effect of operating conditions on the optically induced electrokinetic (OEK)-based manipulation of magnetic microbeads in a microfluidic system
Jia-Long Hong,Chia-Ming Yang,Po-Yu Chu,Wen-Pin Chou,Chia-Jung Liao,Chia-Hsun Hsieh,Min-Hsien Wu,Min-Hsien Wu,Ping-Hei Chen +8 more
TL;DR: In this paper, the effect of operating conditions on the OEK-based magnetic microbead manipulation was investigated and the results showed that the maximum terminal velocity (Vterminal) of a light image that can manipulate micro-beads decreased significantly with increasing AC frequency.
Journal ArticleDOI
Gravitational sedimentation-based approach for ultra-simple and flexible cell patterning coculture on microfluidic device.
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Fabrication of high-aspect-ratio 3D hydrogel microstructures using optically induced electrokinetics
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References
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Journal ArticleDOI
A revolution in optical manipulation
TL;DR: This research presents the next generation of single-beam optical traps, which promise to take optical tweezers out of the laboratory and into the mainstream of manufacturing and diagnostics and even become consumer products.
Journal ArticleDOI
Optical trapping and manipulation of single cells using infrared laser beams
TL;DR: The use of infrared (IR) light is used to make much improved laser traps with significantly less optical damage to a variety of living cells, and new manipulative techniques using IR light are capable of producing large forces under damage-free conditions and improve the prospects for wider use of optical manipulation techniques in microbiology.
Journal ArticleDOI
Separation of Metallic from Semiconducting Single-Walled Carbon Nanotubes
TL;DR: This work has developed a method to separate metallic from semiconducting single-walled carbon nanotubes from suspension using alternating current dielectrophoresis, taking advantage of the difference of the relative dielectric constants of the two species with respect to the solvent.
Journal ArticleDOI
Dynamic holographic optical tweezers
TL;DR: In this article, the authors describe methods for creating large numbers of high-quality optical traps in arbitrary three-dimensional configurations and for dynamically reconfiguring them under computer control, allowing for mixed arrays of traps based on different modes of light, including optical vortices, axial line traps, optical bottles and optical rotators.
Journal ArticleDOI
Microfluidic sorting in an optical lattice
TL;DR: An optical sorter for microscopic particles that exploits the interaction of particles—biological or otherwise—with an extended, interlinked, dynamically reconfigurable, three-dimensional optical lattice, and can be applied in colloidal, molecular and biological research.