Journal ArticleDOI
Massively parallel manipulation of single cells and microparticles using optical images.
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TLDR
An optical image-driven dielectrophoresis technique that permits high-resolution patterning of electric fields on a photoconductive surface for manipulating single particles and requires 100,000 times less optical intensity than optical tweezers is presented.Abstract:
The ability to manipulate biological cells and micrometre-scale particles plays an important role in many biological and colloidal science applications. However, conventional manipulation techniques--including optical tweezers, electrokinetic forces (electrophoresis, dielectrophoresis, travelling-wave dielectrophoresis), magnetic tweezers, acoustic traps and hydrodynamic flows--cannot achieve high resolution and high throughput at the same time. Optical tweezers offer high resolution for trapping single particles, but have a limited manipulation area owing to tight focusing requirements; on the other hand, electrokinetic forces and other mechanisms provide high throughput, but lack the flexibility or the spatial resolution necessary for controlling individual cells. Here we present an optical image-driven dielectrophoresis technique that permits high-resolution patterning of electric fields on a photoconductive surface for manipulating single particles. It requires 100,000 times less optical intensity than optical tweezers. Using an incoherent light source (a light-emitting diode or a halogen lamp) and a digital micromirror spatial light modulator, we have demonstrated parallel manipulation of 15,000 particle traps on a 1.3 x 1.0 mm2 area. With direct optical imaging control, multiple manipulation functions are combined to achieve complex, multi-step manipulation protocols.read more
Citations
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Light-guided electrodeposition of non-noble catalyst patterns for photoelectrochemical hydrogen evolution
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Nanocomposite polymer carbon-black coating for triggering pyro-electrohydrodynamic inkjet printing
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TL;DR: In this article, a light-absorbing polymer nanocomposite thin layer was applied on the ferroelectric substrate to trigger pyro-electrohydrodynamic (EHD) manipulation of liquids.
Journal ArticleDOI
Optofluidic vortex arrays generated by graphene oxide for tweezers, motors and self-assembly
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Journal ArticleDOI
Simultaneous Raman micro–spectroscopy of optically trapped and stacked cells
TL;DR: In this paper, the evolution of the Raman signal intensity with an axial increment of the mass of the substance of interest inside a specific Raman excitation volume is investigated.
Journal ArticleDOI
Precise capture and dynamic relocation of nanoparticulate biomolecules through dielectrophoretic enhancement by vertical nanogap architectures
Eui-Sang Yu,Eui-Sang Yu,Hyojin Lee,Sun-Mi Lee,Jiwon Kim,Taehyun Kim,Jongsu Lee,Chulki Kim,Minah Seo,Jae Hun Kim,Young Tae Byun,Seungchul Park,Seung-Yeol Lee,Sin-Doo Lee,Yong Sang Ryu +14 more
TL;DR: A vertical nanogap architecture with an electrode-insulator-electrode stack structure is proposed, facilitating the generation of strong dielectrophoretic forces at low voltages, to precisely capture and spatiotemporally manipulate nanoparticles and molecular assemblies, including lipid vesicles and amyloid-beta protofibrils/oligomers.
References
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Journal ArticleDOI
A revolution in optical manipulation
TL;DR: This research presents the next generation of single-beam optical traps, which promise to take optical tweezers out of the laboratory and into the mainstream of manufacturing and diagnostics and even become consumer products.
Journal ArticleDOI
Optical trapping and manipulation of single cells using infrared laser beams
TL;DR: The use of infrared (IR) light is used to make much improved laser traps with significantly less optical damage to a variety of living cells, and new manipulative techniques using IR light are capable of producing large forces under damage-free conditions and improve the prospects for wider use of optical manipulation techniques in microbiology.
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Separation of Metallic from Semiconducting Single-Walled Carbon Nanotubes
TL;DR: This work has developed a method to separate metallic from semiconducting single-walled carbon nanotubes from suspension using alternating current dielectrophoresis, taking advantage of the difference of the relative dielectric constants of the two species with respect to the solvent.
Journal ArticleDOI
Dynamic holographic optical tweezers
TL;DR: In this article, the authors describe methods for creating large numbers of high-quality optical traps in arbitrary three-dimensional configurations and for dynamically reconfiguring them under computer control, allowing for mixed arrays of traps based on different modes of light, including optical vortices, axial line traps, optical bottles and optical rotators.
Journal ArticleDOI
Microfluidic sorting in an optical lattice
TL;DR: An optical sorter for microscopic particles that exploits the interaction of particles—biological or otherwise—with an extended, interlinked, dynamically reconfigurable, three-dimensional optical lattice, and can be applied in colloidal, molecular and biological research.