Journal ArticleDOI
Measurement of substance P metabolites in rat CNS.
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TLDR
A procedure based on ion‐exchange chromatography for chemical separation and radioimmunoassays for quantitation of substance P(SP), the SP(1–7), and C‐terminal fragments, respectively, has been developed.Abstract:
A procedure based on ion-exchange chromatography for chemical separation and radioimmunoassays for quantitation of substance P (SP), the SP(1-7), and C-terminal fragments, respectively, has been developed. The procedure allows the determination of these fragments in the presence of large (i.e., 50- to 100-fold) excess of parent compound. The chemical identity of isolated SP and fragments was studied with preparative electrophoresis on dilute agarose gel and with HPLC. The activity identified as SP(1-7) comigrated with the authentic standard whereas practically all activity isolated as C-terminal fragments comigrated with SP(5-11). The levels of C-terminal fragments in rat brain areas rich in SP and in spinal cord were 1-2% of those of parent compound. The levels of SP(1-7) were always higher, in the spinal cord markedly higher (three to five times). Postmortem storage of samples from brain and spinal cord indicated that SP(1-7) levels fell more rapidly than those of SP or C-terminal fragments.read more
Citations
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Journal ArticleDOI
Calcitonin gene-related peptide is a potent inhibitor of substance P degradation.
TL;DR: Calcitonin gene-related peptide was found to potently inhibit a substance P endopeptidase isolated from human CSF, suggesting a possible mechanism is interaction for a common metabolic step.
Journal ArticleDOI
Tachykinin receptors: a radioligand binding perspective.
TL;DR: Current evidence suggests that tachykinin coexistence and expression of multiple receptors may also occur with postulated NK-2 and NK-1 receptor subtypes, and the convenient working hypothesis of three endogenous ligands for three basic receptor types may be too simplistic and in need of amendment.
Journal ArticleDOI
Striato-nigral dynorphin and substance P pathways in the rat. I. Biochemical and immunohistochemical studies.
I. Christensson-Nylander,Mario Herrera-Marschitz,W. Staines,T. Hökfelt,Lars Terenius,Urban Ungerstedt,Claudio Cuello,W. H. Oertel,Menek Goldstein +8 more
TL;DR: It is concluded that the dynorphin and substance P striatonigral pathways have different functions and Substance P in the striato-nigral pathway may have a role in a positive feed-back loop regulating the firing of nigro-striatal dopamine neurons, while Dynorphin might be important in negative feed- back control.
Journal ArticleDOI
Differential modulation of striatal dopamine release by intranigral injection of γ-aminobutyric acid (GABA), dynorphin A and substance P
TL;DR: The present results support the concept that substance P, directly or indirectly, provides a positive feed-back regulation for the release of striatal DA, whereas GABA and dynorphin exert a negative feed- back regulation.
Journal ArticleDOI
Differential effects of C- and N-terminal substance P metabolites on the release of amino acid neurotransmitters from the spinal cord: potential role in nociception
TL;DR: It is suggested that the N-terminal products of SP metabolism play a distinct role in the modulation of SP(1–11) nociception, possibly through an interaction with an opiate receptor.
References
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Journal ArticleDOI
Substance P and [Leu]enkephalin are hydrolyzed by an enzyme in pig caudate synaptic membranes that is identical with the endopeptidase of kidney microvilli
TL;DR: The endopeptidase is present in caudate synaptic membranes and could play an important role in the hydrolysis of neuropeptides and should be designated "endopePTidase-24".
Journal ArticleDOI
Enhancement of phagocytosis - a newly found activity of substance P residing in its N-terminal tetrapeptide sequence.
Zvi Bar-Shavit,Rachel Goldman,Yitzhak Stabinsky,Philip A. Gottlieb,Mati Fridkin,Vivian I. Teichberg,Shmaryahu Blumberg +6 more
TL;DR: The phagocytosis-enhancing activity of Substance P may play a role in inflammatory processes of neural origin where the involvement of the peptide has been implicated.
Journal ArticleDOI
Neuroleptic activity of the neuropeptide β-LPH62–77 ([Des-Tyr1]γ-endorphin; DTγE)
TL;DR: The results are interpreted to indicate that DTγE or a closely related peptide is an endogenous neuroleptic and may be that a reduced availability as a result of an inborn error in the generation ofDTγE is an etiological factor in psychopathological states for which Neuroleptic drugs are beneficial.
Book ChapterDOI
Substance P in the Nervous System
TL;DR: The properties of a vasodepressor substance extracted from brain and intestine and originally called preparation P were first described by von Euler and Gaddum in 1931 and are surprisingly difficult to cite a single physiologically relevant action of substance P.
Journal ArticleDOI
Purification and properties of a prolyl endopeptidase from rabbit brain.
TL;DR: An enzyme with the specificity of a prolyl endopeptidase was purified about 880‐fold from rabbit brain and hydrolyzed to N‐benzyloxycarbonylglycyl‐L‐proline and free sulfamethoxazole which can be conveniently determined by a colorimetric procedure.