Measuring mechanical anisotropy of the cornea with Brillouin microscopy
TLDR
In this article , the stiffness tensor for a lamellar network of collagen fibrils and angle-resolved Brillouin measurements were used to determine the longitudinal stiffness coefficients describing the ex vivo porcine cornea as a transverse isotropic material.Abstract:
Abstract Load-bearing tissues are typically fortified by networks of protein fibers, often with preferential orientations. This fiber structure imparts the tissues with direction-dependent mechanical properties optimized to support specific external loads. To accurately model and predict tissues’ mechanical response, it is essential to characterize the anisotropy on a microstructural scale. Previously, it has been difficult to measure the mechanical properties of intact tissues noninvasively. Here, we use Brillouin optical microscopy to visualize and quantify the anisotropic mechanical properties of corneal tissues at different length scales. We derive the stiffness tensor for a lamellar network of collagen fibrils and use angle-resolved Brillouin measurements to determine the longitudinal stiffness coefficients (longitudinal moduli) describing the ex vivo porcine cornea as a transverse isotropic material. Lastly, we observe significant mechanical anisotropy of the human cornea in vivo, highlighting the potential for clinical applications of off-axis Brillouin microscopy. read more
Citations
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Recent advances in optical elastography and emerging opportunities in the basic sciences and translational medicine [Invited].
TL;DR: In this paper , a review of optical coherence elastography, Brillouin microscopy, optical microrheology, and photoacoustic elastograph is presented.
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Corneal and Scleral Biomechanics in Ophthalmic Diseases: An Updated Review
Yang Wang,Huazheng Cao +1 more
TL;DR: In this article , the role of corneal and scleral biomechanics in ophthalmology and its clinical translation is highlighted, and advances and prospects in cornea and SCLal biomechanical properties and possible associated diseases are addressed.
Journal ArticleDOI
Multiple Optical Elastography Techniques Reveal the Regulation of Corneal Stiffness by Collagen XII
Achuth Nair,Yogeshwari S. Ambekar,Christian Zevallos-Delgado,Taye Tolu Mekonnen,Mei Sun,Fernando Zvietcovich,Manmohan Singh,Salavat R. Aglyamov,Manuel Koch,Giuliano Scarcelli,Edgar M. Espana,Kirill V. Larin +11 more
TL;DR: In this paper , the effect of collagen XII deficiency on the stiffness of the murine cornea was investigated using three-prong optical elastography approach to investigate the mechanical properties of the cornea.
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Regularization of dielectric tensor tomography.
TL;DR: In this article , the application of total variation and positive semi-definiteness regularization to three-dimensional tensor distributions was demonstrated. But the reduction of artifacts was limited to dielectric tensor tomography.
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High-speed non-contact measurement of elasto-optic coefficient via laser-induced phonons
TL;DR: In this paper , the elasto-optic coefficients of common liquids are measured with only 10 ms and an averaged relative standard deviation of 1.52%, complementing the rare liquid data.
References
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Weak elastic anisotropy
TL;DR: The equations governing weak anisotropy are much simpler than those governing strong anisotropic, and they are much easier to grasp intuitively as discussed by the authors, which is why they are easier to understand intuitively.
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Characterization of the anisotropic mechanical properties of excised human skin
Aisling Ní Annaidh,Karine Bruyere,Michel Destrade,Michel Destrade,Michael D. Gilchrist,Michael D. Gilchrist,Mélanie Ottenio +6 more
TL;DR: The histological investigation concluded that there is a definite correlation between the orientation of the Langer lines and the preferred orientation of collagen fibres in the dermis and the data obtained will provide essential information for those wishing to model the skin using a structural constitutive model.
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Confocal Brillouin microscopy for three-dimensional mechanical imaging
Giuliano Scarcelli,Seok Hyun Yun +1 more
TL;DR: A confocal Brillouin microscope based on a fully parallel spectrometer-a virtually imaged phased array-that improves the detection efficiency by nearly 100-fold over previous approaches is demonstrated and the first cross-sectional BrillouIn imaging based on elastic properties as the contrast mechanism is shown.
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Changes in collagen orientation and distribution in keratoconus corneas
Keith M. Meek,Stephen J. Tuft,Yifei Huang,Paulvinder S. Gill,Sally Hayes,Richard Hugh Newton,Anthony J. Bron +6 more
TL;DR: The development of keratoconus involves a high degree of inter- and probably intralamellar displacement and slippage that leads to thinning of the central cornea and associated changes in corneal curvature and may be promoted by a loss of cohesive forces and mechanical failure in regions where lamellae bifurcate.
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Design and analysis of tissue engineering scaffolds that mimic soft tissue mechanical anisotropy.
TL;DR: This study developed ES-PEUU scaffolds under variable speed conditions and modeled the effects of fiber orientation on the macro-mechanical properties of the scaffold, which will help to provide the basis for rationally designed mechanically anisotropic soft tissue engineered implants.
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