Journal ArticleDOI
Mechanisms of Bacterial Transcription Termination.
TLDR
Bacterial transcription termination occurs in three recognized ways: intrinsic termination, an activity only of the core RNAP enzyme and transcript sequences that encode an RNA hairpin and terminal uridine-rich segment; termination by the enzyme Rho, an ATP-dependent RNA translocase that releases RNA by forcing uncharacterized structural changes in the elongating complex.About:
This article is published in Journal of Molecular Biology.The article was published on 2019-04-09. It has received 96 citations till now. The article focuses on the topics: Intrinsic termination & Rho factor.read more
Citations
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Journal ArticleDOI
Trouble is coming: Signaling pathways that regulate general stress responses in bacteria
TL;DR: The modes of signaling to and levels of regulation of the E. coli general Stress Response are discussed and are used as a basis for comparison with the general stress response in other bacteria with the aim of extracting key principles that are common among different species and highlighting important unanswered questions.
Journal Article
Shortening of RNA:DNA Hybrid in the Elongation Complex of RNA Polymerase Is a Prerequisite for Transcription Termination: DNA Hybrid in the Elongation Complex of RNA Polymerase Is a Prerequisite for Transcription Termination
TL;DR: In this paper, it was shown that folding of the hairpin disrupts the three upstream base pairs of the 8 bp RNA:DNA hybrid, a major stability determinant in the complex.
Journal ArticleDOI
Structural basis of transcription-translation coupling.
Chengyuan Wang,Vadim Molodtsov,Emre Firlar,Jason T. Kaelber,Gregor Blaha,Min Su,Richard H. Ebright +6 more
TL;DR: Cryo–electron microscopy structures of Escherichia coli transcription-translation complexes (TTCs) containing different-length mRNA spacers between RNAP and the ribosome active-center P site are reported and it is proposed that TTC-B mediates NusG- and NusA-dependent transcription- translation coupling.
Journal ArticleDOI
Regulation of Transcription Termination of Small RNAs and by Small RNAs: Molecular Mechanisms and Biological Functions.
TL;DR: Current knowledge on intrinsic termination of sRNAs and sRNA-mediated Rho-dependent termination of protein coding genes in bacteria is reviewed.
Journal ArticleDOI
Transcription of Bacterial Chromatin
Beth A. Shen,Robert Landick +1 more
TL;DR: Current understanding of transcription of bacterial chromatin is reviewed with a focus on gene silencing by H-NS, transcription coordination by HU, and potential phase separation by Dps, to anticipate answers that will define how bacterial Chromatin helps coordinate and dynamically regulate gene expression in changing environments.
References
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Journal ArticleDOI
Structural Basis of Transcription: An RNA Polymerase II Elongation Complex at 3.3 Å Resolution
TL;DR: The crystal structure of RNA polymerase II in the act of transcription was determined at 3.3 Å resolution and protein–nucleic acid contacts help explain DNA and RNA strand contacts, the specificity of RNA synthesis, “abortive cycling” during transcription initiation, and RNA and DNA translocation during transcription elongation.
Journal ArticleDOI
Crystal structure of a bacterial RNA polymerase holoenzyme at 2.6 A resolution
Dmitry G. Vassylyev,Shun-ichi Sekine,Oleg Laptenko,Jookyung Lee,Marina N. Vassylyeva,Sergei Borukhov,Shigeyuki Yokoyama +6 more
TL;DR: The crystal structure of a bacterial RNA polymerase holoenzyme from Thermus thermophilus at 2.6 Å resolution provides insight into the structural organization of transcription intermediate complexes and into the mechanism of transcription initiation.
Journal ArticleDOI
Termination Factor for RNA Synthesis
TL;DR: A new protein has been isolated from E. coli which causes specific termination and release of RNA during synthesis in vitro and has been given the name ρ-factor.
Journal ArticleDOI
Molecular mechanism of transcription-repair coupling
Christopher P. Selby,Aziz Sancar +1 more
TL;DR: Mfd appears to target the transcribed strand for repair by recognizing a stalledRNAP and actively recruiting the repair enzyme to the transcription blocking lesion as it dissociates the stalled RNAP.
Journal ArticleDOI
Control of Transcription Termination
Sankar Adhya,Max E. Gottesman +1 more
TL;DR: The author revealed that RNA polymerase mutations located at the Site of Termination Site are linked to RHO mutations, and that pN acts at Many Sites and recognition Specificity of pN is recognized.