Membrane Glycoproteins: I. ENZYMATIC SYNTHESIS OF MANNOSYL PHOSPHORYL POLYISOPRENOL AND ITS ROLE AS A MANNOSYL DONOR IN GLYCOPROTEIN SYNTHESIS
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TLDR
Results indicate that the mannosyl lipid formed in hen oviduct and bovine thyroid catalyze the transfer of mannose from GDP-mannose to endogenous acceptors has the structure of mannosy phosphoryl polyisoprenol; the precise chain length of the poly isoprenyl moiety remains to be established.About:
This article is published in Journal of Biological Chemistry.The article was published on 1973-11-10 and is currently open access. It has received 183 citations till now. The article focuses on the topics: Proteolytic enzymes.read more
Citations
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Mechanism of action of tunicamycin on the UDP-GlcNAc:dolichyl-phosphate GlcNAc-1-phosphate transferase
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The role of lipid intermediates in the glycosylation of proteins in the eucaryotic cell.
Armando J. Parodi,Luis F. Leloir +1 more
Journal ArticleDOI
Evidence for the participation of saccharide-lipids in the synthesis of the oligosaccharide chain of ovalbumin.
D K Struck,W J Lennarz +1 more
TL;DR: Results indicate that saccharide-lipids participate in the assembly of the core oligosaccharide of the secretory glycoprotein, ovalbumin, and that tunicamycin administered in vivo depletes endogenous pools of N-acetylglucosamine-lipid precursors to oligosACcharide -lipid in a manner consistent with its activity in vitro.
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Processing of high mannose oligosaccharides to form complex type oligosaccharides on the newly synthesized polypeptides of the vesicular stomatitis virus G protein and the IgG heavy chain.
TL;DR: Experiments performed with mouse LPCl cells which secrete IgG demonstrated a similar sequence of processing of the oligosaccharide units of this immunoglobulin, indicating that processing is probably a general phenomenon.
References
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Journal Article
Protein Measurement with the Folin Phenol Reagent
TL;DR: Procedures are described for measuring protein in solution or after precipitation with acids or other agents, and for the determination of as little as 0.2 gamma of protein.
Journal ArticleDOI
Phosphorus Assay in Column Chromatography
TL;DR: If the highest accuracy was not required, the following manipulations simplified and speeded multiple total phosphorus determinations on the eluates from column chromatographic separations.
Journal ArticleDOI
The biosynthesis of mannosyl-1-phosphoryl-polyisoprenol in Micrococcus lysodeikticus and its role in mannan synthesis.
TL;DR: All the available evidence indicates that the structure of this compound is mannosyl-l-phosphoryl, which is likely to be a-D-mannosyl-(1 - 3')-diglyceride.
Journal ArticleDOI
The role of polyprenol-bound saccharides as intermediates in glycoprotein synthesis in liver.
TL;DR: It is reported that liver microsomes catalyze the transfer of the oligosaccharide from the glucosylated endogenous acceptor to an endogenous protein, which requires the presence of bivalent cations, manganese being more effective than magnesium.
Journal ArticleDOI
Glucose Transfer from Dolichol Monophosphate Glucose: The Product Formed with Endogenous Microsomal Acceptor
TL;DR: It is tentatively suggested that the compound is dolichol joined through a phosphate or pyrophosphate bridge to an oligosaccharide containing about 20 monosACcharide residues.
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