Membrane Potential Determined by Flow Cytometry Predicts Fertilizing Ability of Human Sperm.
Lis C. Puga Molina,Stephanie Gunderson,Joanne Riley,Pascale Lybaert,Aluet Borrego-Alvarez,Emily S. Jungheim,Celia Cm Santi +6 more
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Measurements of human sperm membrane potential could be used to diagnose men with idiopathic infertility and predict IVF success in normozoospermic infertile patients and hyperpolarized human sperm had higher in vitro fertilization (IVF) ratios and higher percentages of acrosomal exocytosis and hyperactivated motility than depolarized sperm.Abstract:
Infertility affects 10 to 15% of couples worldwide, with a male factor contributing up to 50% of these cases. The primary tool for diagnosing male infertility is traditional semen analysis, which reveals sperm concentration, morphology, and motility. However, 25% of infertile men are diagnosed as normozoospermic, meaning that, in many cases, normal-appearing sperm fail to fertilize an egg. Thus, new information regarding the mechanisms by which sperm acquire fertilizing ability is needed to develop a clinically feasible test that can predict sperm function failure. An important feature of sperm fertilization capability in many species is plasma membrane hyperpolarization (membrane potential becoming more negative inside) in response to signals from the egg or female genital tract. In mice, this hyperpolarization is necessary for sperm to undergo the changes in motility (hyperactivation) and acrosomal exocytosis required to fertilize an egg. Human sperm also hyperpolarize during capacitation, but the physiological relevance of this event has not been determined. Here, we used flow cytometry combined with a voltage-sensitive fluorescent probe to measure absolute values of human sperm membrane potential. We found that hyperpolarization of human sperm plasma membrane correlated positively with fertilizing ability. Hyperpolarized human sperm had higher in vitro fertilization (IVF) ratios and higher percentages of acrosomal exocytosis and hyperactivated motility than depolarized sperm. We propose that measurements of human sperm membrane potential could be used to diagnose men with idiopathic infertility and predict IVF success in normozoospermic infertile patients. Patients with depolarized values could be guided toward intracytoplasmic sperm injection, preventing unnecessary cycles of intrauterine insemination or IVF. Conversely, patients with hyperpolarized values of sperm membrane potential could undergo only conventional IVF, avoiding the risks and costs associated with intracytoplasmic sperm injection.read more
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Journal ArticleDOI
Sperm ion channels and transporters in male fertility and infertility.
TL;DR: Improved understanding of spermatozoan ion channels and transporters will help elucidate the delicate and dynamic regulation of Ca 2+ homeostasis in sperm motility and fertility.
Journal ArticleDOI
Flow cytometry: past and future.
TL;DR: Spectral flow cytometry is a single-cell technology that measures scatter and fluorescence to establish a set of unique cellular properties that can identify multiple phenotypic subsets from a mixture, select a single cell and even isolate that cell by a process called cell sorting.
Journal ArticleDOI
Everything you ever wanted to know about PKA regulation and its involvement in mammalian sperm capacitation.
Carolina Baro Graf,Carla Ritagliati,Cintia Stival,Guillermina Maria Luque,Iñaki Gentile,Mariano G. Buffone,Dario Krapf,Dario Krapf +7 more
TL;DR: Recent understanding of regulatory mechanisms of PKA in mammalian sperm, such as post-translational modifications, in the context of its role as the master orchestrator of molecular events conducive to capacitation is discussed.
Journal ArticleDOI
Membrane Potential Assessment by Fluorimetry as a Predictor Tool of Human Sperm Fertilizing Capacity.
Carolina Baro Graf,Carolina Baro Graf,Carla Ritagliati,Valentina Torres-Monserrat,Cintia Stival,Carlos Carizza,Mariano G. Buffone,Dario Krapf,Dario Krapf +8 more
TL;DR: Results show that Em hyperpolarization could be an indicator of human sperm fertilizing capacity, setting the basis for the use of Em values as a robust predictor of the success rate of IVF.
Journal ArticleDOI
Machine-learning algorithm incorporating capacitated sperm intracellular pH predicts conventional in vitro fertilization success in normospermic patients.
Stephanie Gunderson,Lis C. Puga Molina,Nicholas C. Spies,Paula A. Balestrini,Mariano G. Buffone,Emily S. Jungheim,Joan K. Riley,Celia M. Santi +7 more
TL;DR: In this article, a machine learning algorithm was used to predict successful conventional in vitro fertilization (IVF) in normospermic patients undergoing IVF, defined as the number of fertilized oocytes [2 pronuclei]/number of mature oocytes greater than 0.66.
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