Modified time–kill assay against multidrug-resistant Enterococcus faecium with novel antimicrobial combinations
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This study used a modified time-kill assay to compare the in-vitro activity of chloramphenicol and quinopristin/dalfopristsin combined with vancomycin, ampicillin or gentamicin against multidrug-resistant Enterococcus faecium.Abstract:
This study used a modified time-kill assay to compare the in-vitro activity of chloramphenicol and quinopristin/dalfopristin combined with vancomycin, ampicillin or gentamicin against multidrug-resistant Enterococcus faecium. The assay uses standardized time-kill methods with the following modifications: centrifugation of the test tubes at 1-2 h intervals, removal of supernatant and resuspension of bacteria in media containing antibiotic concentrations corresponding to simulated steady-state serum concentrations. None of the agents, alone or in combination, produced bactericidal or synergic activity. The modified time-kill assay more closely simulates in-vivo conditions and may provide a better qualitative assay to determine the interaction between antimicrobial agents and bacteria.read more
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Treatment of Meningitis Caused by Vancomycin-Resistant Enterococcus faecium: High-Dose and Combination Daptomycin Therapy
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Combining Quinupristin/Dalfopristin with Other Agents for Resistant Infections
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References
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Journal ArticleDOI
National Committee for Clinical Laboratory Standards.
TL;DR: Many members of the Academy of Pediatrics seem to be generally unaware of the fact that the Academy has participated for ten years in a very interesting and valuable organization, the National Committee for Clinical Laboratory Standards (NCCLS).
Journal ArticleDOI
Pharmacokinetics of vancomycin
TL;DR: It appears that excessively high serum concentrations of vancomycin may be associated with ototoxicity, and it has not been possible to relate the development of nephrotoxicity to determined previously serum concentrations.
Journal Article
Studies on the pathogenesis of meningitis. VI. Antagonism between penicillin and chloramphenicol in experimental pneumococcal meningitis.
Journal ArticleDOI
Clinical pharmacology of ampicillin and hetacillin.
TL;DR: Hetacillin, a related compound, contains the 6-amino group as part of an imidazolidinone ring, and this compound can be made by the reaction of acetone with ampicillin.
Journal ArticleDOI
In Vitro Susceptibility of Shigella Strains to Trimethoprim and Sulfamethoxazole
TL;DR: Based on these in vitro observations, trimethoprim and sulfamethoxazole appear worth evaluating for treatment of shigellosis due to multiply antibiotic-resistant strains.