Molecular Characterization of the R Factors Implicated in the Carbenicillin Resistance of a Sequence of Pseudomonas aeruginosa Strains Isolated from Burns
TLDR
A single R-factor type may have been maintained in the Burns Unit between the two Pseudomonas outbreaks as a plasmid conferring resistance to ampicillin in K. aerogenes.Abstract:
An outbreak of R-factor-mediated carbenicillin resistance in Pseudomonas aeruginosa in burned patients in March 1969 was followed by a second outbreak 6 months later. No R-factor-carrying P. aeruginosa strains were detected in the intervening period but R-factor-determined lactamase was commonly encountered, particularly in Klebsiella aerogenes strains. A comparison of the molecular properties of the R factors in pseudomonads from the first and second phases with those in the Klebsiella strains from the intervening period showed them to be very closely related. A single R-factor type therefore may have been maintained in the Burns Unit between the two Pseudomonas outbreaks as a plasmid conferring resistance to ampicillin in K. aerogenes .read more
Citations
More filters
Journal ArticleDOI
Replication of an origin-containing derivative of plasmid RK2 dependent on a plasmid function provided in trans.
TL;DR: Results demonstrate that the potentially lethal function specified by fragment B of RK2 is not necessary for replication and that at least one trans-acting function is directly involved in RK 2 replication.
Journal ArticleDOI
Broad host range DNA cloning system for gram-negative bacteria: construction of a gene bank of Rhizobium meliloti.
TL;DR: A broad host range cloning vehicle that can be mobilized at high frequency into Gram-negative bacteria has been constructed from the naturally occurring antibiotic resistance plasmid RK2.
Journal ArticleDOI
Identification and classification of bacterial plasmids.
TL;DR: A bank of hybrid plasmids carrying probes for replicons is constructed and Nick-translational labeling of probe DNA with biotin and colorimetric detection of hybridization is described.
Journal ArticleDOI
Genomics of IncP-1 antibiotic resistance plasmids isolated from wastewater treatment plants provides evidence for a widely accessible drug resistance gene pool
TL;DR: Findings derived from genomic analysis of IncP-1 resistance plasmids isolated from WWTP bacteria reveal that animal, human and plant pathogens and other bacteria isolated from different habitats share a common pool of resistance determinants.
References
More filters
Journal ArticleDOI
Sedimentation studies of the size and shape of dna.
TL;DR: Analytical zone sedimentation ( Vinograd, Bruner, Kent & Weigle, 1963) is shown to be a sensitive and reliable method for detecting differences in conformation and for determining the molecular weight of homogeneous phage DNA's.
Journal ArticleDOI
Determination of the base composition of deoxyribonucleic acid from its buoyant density in CsCl.
TL;DR: A comprehensive study of the buoyant density of DNA as a function of composition has been made and the linear relation previously reported has been confirmed.
Book ChapterDOI
The β-Lactamases of Gram-Negative Bacteria and their Possible Physiological Role
M H Richmond,Richard B. Sykes +1 more
TL;DR: There is complexity in breakdown of the cephalosporins after β -lactamase action as there is no sporin and the formation of any single product—a fact that invalidates the iodometric assay of cep Halosporinase for absolute measurement.
Journal ArticleDOI
Properties of an R Factor from Pseudomonas aeruginosa
TL;DR: The R factor was transferable from E. coli to bacterial genera outside the Enterobacteriaceae (Pseudomonas and members of the Rhizobiaceae) to which transfer of F-like and I-like plasmids could not be demonstrated.
Journal ArticleDOI
Properties of RP4, an R Factor Which Originated in Pseudomonas aeruginosa S8
TL;DR: RP1, a group of genes specifying resistance to carbenicillin, neomycin, kanamycin, and tetracycline and originating in a strain of Pseudomonas aeruginosa, was freely transmissible between strains of P. aerug inosa, Escherichia coli, and Proteus mirabilis.