Molecular cloning of human prostate specific antigen cDNA
Åke Lundwall,Hans Lilja +1 more
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TLDR
A λgt11 clone encoding prostate specific antigen has been isolated from a human prostate cDNA library and the primary structure shows extensive homology with proteases of the kallikrein family.About:
This article is published in FEBS Letters.The article was published on 1987-04-20 and is currently open access. It has received 449 citations till now. The article focuses on the topics: cDNA library & Complementary DNA.read more
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Prostate specific antigen: a critical assessment of the most useful tumor marker for adenocarcinoma of the prostate.
TL;DR: It is unlikely that PSA by itself will become an effective screening tool for the early diagnosis of prostate cancer, but if combined with digital rectal examination and/or transrectal ultrasound it may become a vital part of any early detection program.
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Biology of prostate-specific antigen.
TL;DR: Serum total PSA levels are increased in PCa, and PSA screening has dramatically altered PCa presentation and management, and recent data indicate that there may be additional roles for PSA in the pathogenesis of PCa.
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Serum prostate specific antigen complexed to alpha 1-antichymotrypsin as an indicator of prostate cancer.
Anders Christensson,Thomas Björk,Olle Nilsson,Ulrika Dahlén,Marja-Terttu Matikainen,Abraham T.K. Cockett,Per-Anders Abrahamsson,Hans Lilja +7 more
TL;DR: The combination of assays measuring total PSA immunoreactivity, the noncomplexed molecular form and PSA in complex with alpha 1-antichymotrypsin may facilitate discrimination between prostate cancer and BPH.
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Enzymatic activity of prostate-specific antigen and its reactions with extracellular serine proteinase inhibitors.
TL;DR: Prostate-specific antigen is one of the three most abundant prostatic-secreted proteins in human semen and when added to normal blood plasma in vitro, active PSA formed stable complexes both with alpha 2-macroglobulin and alpha 1-antichymotrypsin.
Journal Article
Acceleration of human prostate cancer growth in vivo by factors produced by prostate and bone fibroblasts.
TL;DR: Observations illustrate that fibroblasts differentially modulate prostate cancer growth through the release of paracrine-mediated GFs, possibly including bFGF, and that tumor-stromal cell interactions play an important role in prostate cancer grow and progression.
References
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Book
Molecular Cloning: A Laboratory Manual
TL;DR: Molecular Cloning has served as the foundation of technical expertise in labs worldwide for 30 years as mentioned in this paper and has been so popular, or so influential, that no other manual has been more widely used and influential.
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Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease.
TL;DR: In this article, the rat pancreas RNA was used as a source for the purification of alpha-amylase messenger ribonucleic acid (RBA) using 2-mercaptoethanol.
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A simple and very efficient method for generating cDNA libraries
Ueli Gubler,Beth J. Hoffman +1 more
TL;DR: Using the fully sequenced 1300 nucleotide-long bovine preproenkephalin mRNA, it is established by sequencing that the method yields faithful full-length transcripts.
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RNA molecular weight determinations by gel electrophoresis under denaturing conditions, a critical reexamination.
TL;DR: RNA molecular weight measurements were carried out by gel electrophoresis under four different denaturing conditions including 99% formamide, 10 mM methyl mercury, 2.2 M formaldehyde, and 6 M urea at pH 3.8 to demonstrate reliable molecular weight determinations of denatured RNAs, especially those obtained by extrapolation.
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Efficient isolation of genes by using antibody probes.
Richard A. Young,Ronald W. Davis +1 more
TL;DR: A sensitive and general technique has been devised for the dual purposes of cloning genes by using antibodies as probes and isolating unknown proteins encoded by cloned DNA using an expression vector that permits insertion of foreign DNA into the beta-galactosidase structural gene lacZ and promotes synthesis of hybrid proteins.