Journal ArticleDOI
Molecular Identification of Helicoverpa armigera (Lepidoptera: Noctuidae: Heliothinae) in Argentina and Development of a Novel PCR-RFLP Method for its Rapid Differentiation From H. zea and H. gelotopoeon
TLDR
The data reported here constitute the first molecular confirmation of this pest in the country, and reveal the occurrence of H. armigera in northern and central Argentina, including the main soybean- and maize-producing area.Abstract:
Helicoverpa armigera (Hubner) (Lepidoptera: Noctuidae: Heliothinae) is among the most voracious global pests of agriculture. Adults of this species were identified recently in northern Argentina by dissection of male genitalia. In this work, a rapid and simple molecular tool was designed to distinguish H. armigera from the morphologically similar indigenous bollworms Helicoverpa zea (Boddie) and Helicoverpa gelotopoeon (Dyar), regardless of the life stage. Amplification of partial COI gene with a new primer pair, and subsequent digestion with endonuclease HinfI, yielded different RFLP profiles for the three main Helicoverpa pests currently present in South America. The method was validated in Helicoverpa specimens collected across Argentina, whose identity was further corroborated by COI sequencing and phylogenetic analysis. The data reported here constitute the first molecular confirmation of this pest in the country. The survey revealed the occurrence of H. armigera in northern and central Argentina, including the main soybean- and maize-producing area.read more
Citations
More filters
Journal ArticleDOI
Current situation of pests targeted by Bt crops in Latin America
Carlos A. Blanco,W Chiaravalle,M Dalla-Rizza,Juliano Ricardo Farias,MF García-Degano,Gerardo Alfredo Gastaminza,David Mota-Sanchez,María Gabriela Murúa,Celso Omoto,BK Pieralisi,J. C. Rodríguez,JC Rodríguez-Maciel,H Terán-Santofimio,Antonio P. Terán-Vargas,SJ Valencia,Eduardo Willink +15 more
TL;DR: Of the 31 pests targeted and controlled by Bt crops in Latin America, only S. frugiperda has shown tolerance to certain Bt proteins in growers' fields, the most reliable indication of the status of Bt-susceptibility in most of the American continent.
Journal ArticleDOI
Detection of sister-species in invasive populations of the fall armyworm Spodoptera frugiperda (Lepidoptera: Noctuidae) from Uganda.
Michael Otim,Wee Tek Tay,Tom Walsh,Dalton Kanyesigye,Stella A. Adumo,Joseph Abongosi,Stephen Ochen,Julius Pyton Sserumaga,Simon Alibu,Grace Abalo,Godfrey Asea,Ambrose Agona +11 more
TL;DR: Knowledge of the FAW genetic diversity will be needed to assess the risks of introducing Bt-resistance traits and to understand theFAW incursion pathways into the Old World and its potential onward spread.
Journal ArticleDOI
Mitochondrial DNA COI characterization of Helicoverpa armigera (Lepidoptera: Noctuidae) from Paraguay and Uruguay.
Jonas Andre Arnemann,W.J. James,Tom Walsh,Jerson Vanderlei Carús Guedes,Guy Smagghe,E. Castiglioni,Wee Tek Tay +6 more
TL;DR: Evidence is presented of extending the South American range of H. armigera to Uruguay, using polymerase chain reaction and sequencing of the partial mitochondrial DNA (mtDNA) cytochrome oxidase I region, which supports previous morphological identification of the Old World cotton bollworm in Paraguay.
Journal ArticleDOI
Species From the Heliothinae Complex (Lepidoptera: Noctuidae) in Tucumán, Argentina, an Update of Geographical Distribution of Helicoverpa armigera.
M. Gabriela Murúa,Lucas Emiliano Cazado,Augusto Sebastián Casmuz,M. Inés Herrero,M. Elvira Villagrán,Alejandro Vera,Daniel R. Sosa-Gómez,Gerardo Alfredo Gastaminza +7 more
TL;DR: This study determined population fluctuations of the Heliothinae complex in soybean and chickpea crops using male moths collected in pheromone traps in Tucuman province, and update the geographical distribution of H. armigera in Argentina.
Journal ArticleDOI
Overview of Pest Status, Potential Risk, and Management Considerations of Helicoverpa armigera (Lepidoptera: Noctuidae) for U.S. Soybean Production
TL;DR: The overview of the potential threats which H. armigera poses to soybean production in Mid-Southern states when it establishes in the United States is discussed.
References
More filters
Journal ArticleDOI
MEGA5: Molecular Evolutionary Genetics Analysis using Maximum Likelihood, Evolutionary Distance, and Maximum Parsimony Methods
Koichiro Tamura,Daniel S. Peterson,Nicholas Peterson,Glen Stecher,Masatoshi Nei,Sudhir Kumar +5 more
TL;DR: The newest addition in MEGA5 is a collection of maximum likelihood (ML) analyses for inferring evolutionary trees, selecting best-fit substitution models, inferring ancestral states and sequences, and estimating evolutionary rates site-by-site.
Journal ArticleDOI
A brave new world for an old world pest: Helicoverpa armigera (Lepidoptera: Noctuidae) in Brazil.
Wee Tek Tay,Miguel F. Soria,Tom Walsh,Danielle Thomazoni,Pierre Silvie,Gajanan T. Behere,Craig Anderson,Sharon Downes +7 more
TL;DR: Molecular evidence via mitochondrial DNA (mtDNA) cytochrome oxidase I and Cyt b partial gene sequences for the successful recent incursion of H. armigera into the New World is provided, showing that at least two matrilines are present in Brazil.
Journal ArticleDOI
Primeiro registro de ocorrência de Helicoverpa armigera (Hübner) (Lepidoptera: Noctuidae) no Brasil
Cecilia Czepak,Karina Cordeiro Albernaz,Lúcia Madalena Vivan,Humberto Oliveira Guimarães,Tiago Carvalhais +4 more
TL;DR: The first occurrence, in Brazil, of Helicoverpa armigera (Hubner) (Lepidoptera: Noctuidae), which was considered up till now a quarantine pest, is reported.
Journal ArticleDOI
The potential distribution of invading Helicoverpa armigera in North America: Is it just a matter of time?
Darren J. Kriticos,Noboru Ota,William D. Hutchison,Jason M. Beddow,Tom Walsh,Wee Tek Tay,Daniel M. Borchert,Silvana V. Paula-Moraes,Cecilia Czepak,Myron P. Zalucki +9 more
TL;DR: The value of the major host crops in the United States growing within its modelled potential range, including that area where it could expand its range during favourable seasons, was collated and found that the annual value of crops that would be exposed to H. armigera totalled approximately US$78 billion p.a, worth growing in climates that are optimal for the pest.