Journal ArticleDOI
Molecular similarities among the adenovirus-associated virus polypeptides and evidence for a precursor protein.
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It was suggested that VPO could be the primary AAV gene product and that it is processed to VP1, VP2, VP3, and other nonstructural fragments during the AAV replicative cycle.About:
This article is published in Virology.The article was published on 1977-10-01. It has received 26 citations till now. The article focuses on the topics: Gene product.read more
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Book ChapterDOI
Use of adeno-associated virus as a general transduction vector for mammalian cells.
TL;DR: The ability to establish a latent infection which can later be rescued appears to be a mechanism for ensuring the survival of AAV in the absence of a helper virus.
Journal ArticleDOI
Adeno-associated virus general transduction vectors: analysis of proviral structures.
TL;DR: The results suggest that AAV sequences are amplified by DNA replication either before or after integration and that the mechanism of replication is different from the one used during AAV lytic infections.
Journal ArticleDOI
Genetics of adeno-associated virus: isolation and preliminary characterization of adeno-associated virus type 2 mutants.
TL;DR: The constructed insertion and deletion mutants with mutations within the adeno-associated virus (AAV) sequences of the infectious recombinant plasmid pSM620 proves that at least one AAV-coded protein is required for viral DNA replication and suggests the existence of an additional AAV mRNA.
Book ChapterDOI
Adeno-associated viruses: an update.
Kenneth I. Berns,Roy A. Bohenzky +1 more
TL;DR: Adeno-associated virus does not have any structural relatedness to either of its helpers; on the other hand, the three viruses do represent all of the known vertebrate virus families with linear DNA genomes that replicate in cell nuclei.
Journal ArticleDOI
Positive and negative autoregulation of the adeno-associated virus type 2 genome.
TL;DR: The autoregulation of the AAV genome is investigated by analyzing the expression of mutant AAV genomes upon transfection into adenovirus-infected human cells and evidence is presented which suggests that the p5 and p19 genes contain negative cis-active regulatory elements.
References
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Journal Article
Protein Measurement with the Folin Phenol Reagent
TL;DR: Procedures are described for measuring protein in solution or after precipitation with acids or other agents, and for the determination of as little as 0.2 gamma of protein.
Journal ArticleDOI
Molecular weight estimation of polypeptide chains by electrophoresis in SDS-polyacrylamide gels
Journal Article
Application of a microtechnique to viral serological investigations.
TL;DR: A microtechnique (modified Takatsy) is described which can be applied to complement fixation, hemagglutination, hemagenesis inhibition and metabolic inhibition tests andComparative data obtained with the micro- and standard systems establish the reliability and validity of the microsystem.
Journal ArticleDOI
Processing of adenovirus 2-induced proteins
TL;DR: It is proposed that at least two of the adenovirus 2 virion components are derived by the cleavage of higher molecular weight precursor polypeptides, which imply precursor-product relationships for these components.
Journal ArticleDOI
Studies of small DNA viruses found in various adenovirus preparations: physical, biological, and immunological characteristics
TL;DR: The purpose of the present report is to describe some of the physical, biological, and immunological characteristics of the small particles found in various adenovirus preparations with further evidenice that they are defective viruses and not adenOVirus subunits.